Sample result 1
The results are presented in a report containing methods used for the analysis, sample related information, a chromatogram showing the separation of the amino acids, and a component results section containing the amino acid names, retention time, area integrated, and amounts. We can also provide a spreadsheet showing the calculations made if you want your data presented in other units (i.e. in g, g/L, %). A report of the calibration standard run is also included to show the separation of the amino acids and quantity of amino acid used in the standard. The raw data as well as the processed data are kept in our hard drive for five months. After this time, the information is backed up on a CD-R for archiving. The back-up CD-R will be kept for a period of three years.
Archived data can be restored at any time during the three year archival period. A printed copy of the report will be provided
Sample Results
Amino Acid Analysis Facility
SICKKIDS ADVANCED PROTEIN TECHNOLOGY CENTRE
Dept. of Structural Biology & Biochemistry
The Hospital for Sick Children
DATE:__________
Sample Information
Vial: 2 Injection: 1 Injection Volume: 10.00ul Result Id Date Acquired
Sample File Name:
Acq Method Set Hydrolyzates 3B1A Method Set
Investigator:
Date Processed
Processing Method Hydrolyzates 3B1Nleu
Calibration Standard
Pierce Amino Acid Standard H; Derivatized with PITC; Pico-Tag column (3.9 mm X 15 cm); Column Temperature: 38 C; Waters Modified Pico-Tag Gradient; Waters Alliance 2690 Separations Module; Waters 24B7 Dual Wavelength Absorbance Detector; Detection at 254 nm; Waters Millennium 32 Chromatography Software

| Name | RT | Area | Amount |
|---|---|---|---|---|
1 | Asp | 1.597 | 186699 | 250.000 |
2 | Glu | 1.729 | 184636 | 250.000 |
3 | Ser | 2.950 | 180016 | 250.000 |
4 | Gly | 3.183 | 180849 | 250.000 |
5 | His | 3.336 | 194537 | 250.000 |
6 | Arg | 3.747 | 185560 | 250.000 |
7 | Thr | 4.065 | 182778 | 250.000 |
8 | Ala | 4.248 | 191526 | 250.000 |
9 | Pro | 4.457 | 198868 | 250.000 |
10 | NH3 | 4.741 | 101831 | 250.000 |
11 | Tyr | 6.690 | 205595 | 250.000 |
12 | Val | 7.482 | 203020 | 250.000 |
13 | Met | 8.031 | 205064 | 250.000 |
14 | Cys | 9.324 | 120302 | 125.000 |
15 | Ile | 9.543 | 208746 | 250.000 |
16 | Leu | 9.776 | 201256 | 250.000 |
17 | Nleu | 10.214 | 197341 | 250.000 |
18 | RgntPK1 | 10.401 | 40829 | 250.000 |
19 | RgntPk2 | 10.621 | 39621 | 250.000 |
20 | Phe | 10.907 | 206594 | 250.000 |
21 | Lys | 12.016 | 383162 | 250.000 |