Advanced Protein Technology Centre
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Sample result 1

The results are presented in a report containing methods used for the analysis, sample related information, a chromatogram showing the separation of the amino acids, and a component results section containing the amino acid names, retention time, area integrated, and amounts. We can also provide a spreadsheet showing the calculations made if you want your data presented in other units (i.e. in g, g/L, %). A report of the calibration standard run is also included to show the separation of the amino acids and quantity of amino acid used in the standard. The raw data as well as the processed data are kept in our hard drive for five months. After this time, the information is backed up on a CD-R for archiving. The back-up CD-R will be kept for a period of three years.

Archived data can be restored at any time during the three year archival period. A printed copy of the report will be provided

Sample Results

Amino Acid Analysis Facility
SICKKIDS ADVANCED PROTEIN TECHNOLOGY CENTRE
Dept. of Structural Biology & Biochemistry
The Hospital for Sick Children

DATE:__________

Sample Information

Vial: 2 Injection: 1 Injection Volume: 10.00ul Result Id Date Acquired
Sample File Name:
Acq Method Set Hydrolyzates 3B1A Method Set

Investigator:
Date Processed
Processing Method Hydrolyzates 3B1Nleu

Calibration Standard
Pierce Amino Acid Standard H; Derivatized with PITC; Pico-Tag column (3.9 mm X 15 cm); Column Temperature: 38 C; Waters Modified Pico-Tag Gradient; Waters Alliance 2690 Separations Module; Waters 24B7 Dual Wavelength Absorbance Detector; Detection at 254 nm; Waters Millennium 32 Chromatography Software

 

Name

RT

Area

Amount

1

Asp

1.597

186699

250.000

2

Glu

1.729

184636

250.000

3

Ser

2.950

180016

250.000

4

Gly

3.183

180849

250.000

5

His

3.336

194537

250.000

6

Arg

3.747

185560

250.000

7

Thr

4.065

182778

250.000

8

Ala

4.248

191526

250.000

9

Pro

4.457

198868

250.000

10

NH3

4.741

101831

250.000

11

Tyr

6.690

205595

250.000

12

Val

7.482

203020

250.000

13

Met

8.031

205064

250.000

14

Cys

9.324

120302

125.000

15

Ile

9.543

208746

250.000

16

Leu

9.776

201256

250.000

17

Nleu

10.214

197341

250.000

18

RgntPK1

10.401

40829

250.000

19

RgntPk2

10.621

39621

250.000

20

Phe

10.907

206594

250.000

21

Lys

12.016

383162

250.000