Peptide Synthesis
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Synthetic peptides have a wide range of uses including: structure-function analysis, binding assays, receptor agonist/antagonists or immunogens for the production of specific antibodies. The APTC Peptide Synthesis facility was established in 1989 to provide investigators with high quality synthetic peptides at a reasonable price.
The facility owns and operates two flow-through automated single channel synthesizers and one Pioneer multi channel synthetic peptide synthesizer. The three machines employ FMOC Synthetic Chemistry to produce peptides that range in size from several to 100 amino acids at a 0.1 mmole scale and offering the following services:
- Conjugation To Macromolecules: KLH, BSA, Tetanus Toxoid, Lipids, etc.
- Biotinylation: N- or C- Terminus
- Phosphorylation: Phospho-serine, -Threonine, -Tyrosine, -Histidine
- N-Terminal Fluoresceination (FITC)
- Amino Acid Analogues, Unusual Amino Acids, & Mimetics Including Ornithine, L-Malonytyrosine (Non-Hydrolyzable Phospho-Tyrosine Analogue)
- N-Terminus Acetylation/C-Terminus Amidation
Turn around times average 14 days, depending on the application.
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Peptide synthesis remains somewhat of an art. Success depends heavily on the primary sequence of the desired product and the purity of the starting reagents. Dr. Nam-Chaing Wang has over 14 years experience in the synthesis facility and has optimized both reagents and methodologies.