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Amino Acid Analysis Sample Results 1

The results are presented in a report containing methods used for the analysis, sample related information, a chromatogram showing the separation of the amino acids, and a component results section containing the amino acid names, retention time, area integrated, and amounts. We can also provide a spreadsheet showing the calculations made if you want your data presented in other units (i.e. in g, g/L, %). A report of the calibration standard run is also included to show the separation of the amino acids and quantity of amino acid used in the standard. The raw data as well as the processed data are kept in our hard drive for five months. After this time, the information is backed up on a CD-R for archiving. The back-up CD-R will be kept for a period of three years.

Archived data can be restored at any time during the three year archival period. A printed copy of the report will be provided

Sample Results

Amino Acid Analysis Facility
SPARC BioCentre
The Hospital for Sick Children

DATE:__________

Sample Information

Vial: 2 Injection: 1 Injection Volume: 10.00ul Result Id Date Acquired
Sample File Name:
Acq Method Set Hydrolyzates 3B1A Method Set

Investigator:
Date Processed
Processing Method Hydrolyzates 3B1Nleu

Calibration Standard
Pierce Amino Acid Standard H; Derivatized with PITC; Pico-Tag column (3.9 mm X 15 cm); Column Temperature: 38 C; Waters Modified Pico-Tag Gradient; Waters Alliance 2690 Separations Module; Waters 24B7 Dual Wavelength Absorbance Detector; Detection at 254 nm; Waters Millennium 32 Chromatography Software

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 NameRTAreaAmount
1Asp1.597186699250.000
2Glu1.729184636250.000
3Ser2.950180016250.000
4Gly3.183180849250.000
5His3.336194537250.000
6Arg3.747185560250.000
7Thr4.065182778250.000
8Ala4.248191526250.000
9Pro4.457198868250.000
10NH34.741101831250.000
11Tyr6.690205595250.000
12Val7.482203020250.000
13Met8.031205064250.000
14Cys9.324120302125.000
15Ile9.543208746250.000
16Leu9.776201256250.000
17Nleu10.214197341250.000
18RgntPK110.40140829250.000
19RgntPk210.62139621250.000
20Phe10.907206594250.000
21Lys12.016383162250.000