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Amino Acid Analysis Sample Results 2

Amino Acid Analysis Facility
SPARC BioCentre
The Hospital for Sick Children

DATE:_____________




Sample Information

Vial: 5 Injection: 1 Dil_Vol: (uL) 60 Injection Volume: 15 ul Date Acquired
Sample File Name:
Acq Method Set Hydrolyzates 3B1A Method Set

Investigator:
Date Processed
Processing Method Hydrolyzates 3B1Nleu

Calibration Standard
Pierce Amino Acid Standard H; Derivatized with PITC; Pico-Tag column (3.9 mm X 15 cm); Column Temperature: 38 C; Waters Modified Pico-Tag Gradient; Waters Alliance 2690 Separations Module; Waters 24B7 Dual Wavelength Absorbance Detector; Detection at 254 nm; Waters Millennium 32 Chromatography Software

Click to view full-sized image.

 

 

Name

RT

Area

Amount

1

Asp

1.591

25679

137.540

2

Glu

1.713

421318

2281.884

3

Ser

2.910

70541

391.859

4

Gly

3.138

2796510

15463.197

5

Thr

4.021

23624

129.248

6

Ala

4.192

3819360

19941.699

7

Pro

4.406

1582858

7959.346

8

NH3

4.712

120234

1180.729

9

Tyr

6.641

135482

658.973

10

Val

7.405

2354586

11597.781

11

Ile

9.494

269875

1292.841

12

Leu

9.723

255269

1268.381

13

Nieu

10.134

2100363

10643.300

14

RgntPK1

10.345

18021

441.372

15

RgntPK2

10.565

533940

13476.126

16

Phe

10.826

271322

1313.307

17

Lys

12.000

668475

1744.626