FISH - BCR-ABL1
Clinical Significance
t(9;22)(q34;q11.2)
Test Name
FISH - BCR-ABL1
Gene Name
- BCR-ABL1
Division
Cytogenetics
Method
Direct preparation and unstimulated 24 hour culture; FISH (Fluorescence in situ hybridization)
External Proficiency Testing
CAP
QMPLS
Turn Around Time
5-9 working days
Disease/Condition
Acute lymphoblastic leukemia or Chronic myeloid leukemia
Specimen Type
Bone Marrow Aspirate;
or Peripheral Blood
Minimum Specimen Requirements
1.5mL (bone marrow aspirate); 1-3mL (0.5 mL minimum for neonates) (peripheral blood)
Storage/Transportation
Maintain at room temperature. Do not refrigerate or freeze. Ship sample overnight at room temperature for receipt at SickKids Cytogenetics Laboratory within 24 hours of collection.
Special Requirements
Collect sample in sodium heparin.
For aspirate: relocate needle prior to sampling if possible.
If the sample is a new diagnosis leukemia, and is less than the recommended volume, submit peripheral blood in sodium heparin in addition to bone marrow aspirate
Shipping and Contact Information
The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto,
ON
M5G 2G3
Canada
Phone: 416-813-7200
Phone: 1-855-381-3212
Interpretation
Provided