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Default LSRII Fluorochrome Configuration

488nm Laser (Big BLUE 100mW)

  BP LP Fluorochrome
Detector A 780/60 735LP PE-CY7
Detector B 695/40 685LP PE-CY5.5 *
Detector C 610/20 600LP PE-Texas Red
Detector D 575/26 550LP PE
Detector E 530/30 505LP FITC
Detector F empty 505LP none
Detector G 488/10 empty SSC
633nm Laser (RED) BP LP Fluorochrome
Detector A 780/60 735LP APC-CY7
Detector B 710/50 685LP Alexa 700
Detector C 660/20   APC
405nm Laser (Violet) BP LP Fluorochrome
Detector A 560/20 545LP QDOT 565
Detector B 525/50 505LP AmCyan
Detector C empty   Pacific Blue
355 nm UV Laser BP LP Fluorochrome
Detector A 630/30 600LP Hoechst Red
Detector B 424/44 405LP Hoechst Violet

Note 1: to detect PE- Cy5 together with PE-Texas Red, reconfigure the filters for Detector B: replace the 695/40 BP filter with the 635LP and replace the 685LP with the 675LP.

Note 2: to detect PE-Cy5.5 together with PE-Cy5, reconfigure the filters for Detector C: replace the 610/20 BP filter with the 675LP.

Note 3: The filter/PMT configurations are flexible, and this Default Configuration can be changed to suit specific needs.

Please consult the Fluorochrome Excitation/Emission Table and Laser/Filter Combination Table to design your experiment. Check with FCF staff to verify that we have the filters you need for your experiment.If you change filters, please change back to default configuration. Thanks!!!