Tools and Links
Companies
Technotes ![]()
- Tips on Cell Preparation for Flow Cytometric Analysis and Sorting
- Selecting Reagents for Multicolor Flow Cytometry
- Establishing Optimum Baseline PMT Gains to Maximize Resolution
- Hints for working with Diva FCS 3.0 files in FlowJo Workspaces
Presentations ![]()
- OICR Workshop on Flow Cytometry in Cancer Stem Cell Research
- Compensation Workshop
- 091207 FCF Users Group Meeting - Biosafety Policies and PMT Optimization [10 mgs]
- 100222 FCF Users Group Meeting - Methods for Optimizing PMT Voltages
Flow Sites
Online Tutorials & Tools for Flow Cytometry
- Fluorescence Spectra Viewer
- Invitrogen You can access two useful tutorials from this site:
"Introduction to Flow Cytometry" and "Data Analysis" - Access Flow Cytometry Textbook Online
- Multicolor Flow Cytometry Reagent Selection Guide
- Flow Cytometry Worksheet for 3 Laser (blue/red/violet) Experiments

- Flow Cytometry Worksheet for 3 Laser (blue/red/violet) Experiments

- Using Bi-exponential Transformations

Papers to Consider
- Seventeen-colour flow cytometry: unravelling the immune system.
- Interpreting flow cytometry data: a guide for the perplexed.
- A practical approach to multicolor flow cytometry for immunophenotyping.
- Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry.
- A new "Logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data.
- Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry.
- Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats.
An interesting link on the Apple website about Flow Cytometry in research:
Software
