Acknowledgment

The FMCF receives operating funds from sources who periodically ask us to cite publications reporting data from our facility. full policy

To help keep user fees low please acknowledge our contribution in your publications. Then notify us so we can document these publications.

Please email references to

Feedback Survey

Your feedback is the single best way to help us improve our services.

You will be able to access the Sorting Feedback Survey through our online reservation portal and from within sorting appointment confirmation emails.

We look forward to hearing from you.

Mass Cytometry

Getting started

General Information Before You Start

Analytical mass cytometry is only offered as an operated assisted service. Before you start, an initial consultation meeting with the operator is required. To schedule a meeting, please contact Greg Chang (). Please review the information below and bring all your questions to the meeting.

  1. Review reagents available
    Visit the Fluidigm website for currently available reagents and metal-tagged antibodies. For our conjugation services, please go here.
  2. Review sample preparation and staining protocols
    Visit the Nolan Lab website for suggested protocols or contact Greg Chang () for our latest SOPs.
  3. Review Data Analysis options
    Data analysis can be done by FlowJo or Cytobank. The FMCF provides site licenses for the flow cytometry data analysis program FlowJo at a reasonable cost. For documentation on using FlowJo X for Helios data analysis click here. To find out more about Cytobank click here.
  4. Review Helios Sample Requirements
    Learn more

To request instrument time, please visit the Mass cytometry iLab booking site