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BD cell sorters

Aria Fusion VBGR

Aria II
Aria II
Aria II
Name Aria Fusion VBGR
Location TMDT Room 2-504
Restricted use FMCF staff operated only
Lasers 405 nm Violet (85 mW)
488 nm Blue (50 mW)
532 nm Green (150 mW)
640 nm Red (100 mW)
# of colours 16 colours (6V/2B+SSC/5G/3R)
Biosafety cabinet Yes
Default configuration "New advanced configuration"

"New Advanced Configuration" (default)

LP BP Fluorochromes
405 nm, 80 mW, DPSS Violet (Coherent)
Detector A 750 LP 780/60 BV786
Detector B 685 LP 710/50 BV711
Detector C 635 LP 660/20 BV650
Detector D 600 LP 610/20 BV605
Detector E 505 LP 525/50
+ notch
BV 510, V500, AmCyan, Fixable Aqua
Detector F - 450/50 BV421, Pacific Blue, Fixable Violet, Sytox Blue, eF450
Detector G - - No detector
Detector H - - No detector
488 nm, 50 mW, Solid State Blue (Coherent)
Detector A 690 LP 720/40 PerCP-Cy5.5, PerCP-eF710
Detector B 505 LP 515/20 Alexa Fluor 488, CFSE, FITC, GFP, BB515
Detector C - 488/10 SSC
532 nm, 150 mW, Green (Coherent)
Detector A 750 LP 780/60 PE-Cy7
Detector B 685 LP 720/40 PE-Cy5.5
Detector C 635 LP 660/20 PE-Cy5, 7AAD, PI (660/20)
Detector D 600 LP 610/20 PE-Texas Red, PI (610/20), PE/Dazzle 594, PE-CF594
Detector E - 575/25 PE
Detector F - - No detector
Detector G - - No detector
Detector H - - No detector
640 nm, 100 mW, HeNe Red (JDS Uniphase)
Detector A 755 LP 780/60 APC-Cy7, APC-Alexa Fluor 750, APC-Alexa Fluor 780, APC-eF780
Detector B 685 710/40 Alexa Fluor 700
Detector C - 660/20 APC, Alexa Fluor 633, Alexa Fluor 647
The "New Advanced Configuration" is the default cytometer configuration and was designed for a specific set of commonly used fluorochromes in which all detectors are used simultaneously. Filters in this configuration may not be optimal for your fluorochrome combination. Additional filters are available.