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Becton Dickinson (BD) analyzers

CantoII-FCF, VBR

BD Canto II
Name CantoII-FCF, VBR
Location TMDT Room 2-203
Restricted use No
Lasers 407 nm Violet (30 mW)
488 nm Blue (20 mW)
633 nm Red (17 mW)
# of colours 8 colours (2V/4B+SSC/2R)
HTS unit Yes
Default configuration "New advanced configuration 20160107"

"New Advanced Configuration 20160107" (default)

LP BP Fluorochromes
407 nm, 30 mW, Solid State Violet (Coherent)
Detector A 502 LP 510/50 AmCyan, Fixable Aqua
Detector B - 450/50 Pacific Blue, eF450, Fixable Violet, Sytox Blue
Detector C - - No detector
488nm, 20 mW, Solid State Blue (Coherent)
Detector A 735 LP 780/60 PE-Cy7
Detector B 635 LP 670LP PE-Cy5, PerCP, PI, PE-Cy5.5, PerCP-Cy5.5
Detector C 556 LP 585/42 PE
Detector D 502 LP 530/30 FITC, Alexa Fluor 488, CFSE
Detector E - 488/10 SSC
Detector F - - No detector
Detector G - - No detector
Detector H - - No detector
633nm, 17 mW, HeNe Red (JDS Uniphase)
Detector A 735 LP 780/60 APC-Cy7
Detector B - 660/20 APC, Alexa Fluor 633, Alexa Fluor 647
Detector C -   No detector

Note: For GFP, a 515/20 filter is optimal.

The "Advanced Configuration" is the default cytometer configuration and was designed for a specific set of commonly used fluorochromes in which all detectors are used simultaneously. Filters in this configuration may not be optimal for your fluorochrome combination. Additional filters are available.