Acknowledgment

The FMCF receives operating funds from sources who periodically ask us to cite publications reporting data from our facility. full policy

To help keep user fees low please acknowledge our contribution in your publications. Then notify us so we can document these publications.

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Feedback Survey

Your feedback is the single best way to help us improve our services.

You will be able to access the Sorting Feedback Survey through our online reservation portal and from within sorting appointment confirmation emails.

We look forward to hearing from you.

BD cell sorters overview

FACSAria

Photo of instrument

BD FACSAria is a benchtop sorter with digital electronics and a fixed-alignment cuvette flow cell that can detect up to 17 colours, for a total of 19 parameters.

The facility houses several Aria sorters at TMDT. See Biosafety Policies for biosafety requirements for sorting.

General features:

Collection devices

  • Two-way sorting into microtubes, 12 x 75 mm, and 15 mL tubes
  • Four-way sorting into microtubes and 12 x 75 mm tubes
  • One-way sorting (including single cell) onto slides and into 6, 24, 48, 96, and 384-well plates and terisaki plates

Sample Input sizes: Microtubes, 12 x 75-mm, and 15-mL tubes

Temperature Control: cooling or warming for collection tubes and sort sample

Nozzles: (nozzle should be 4-5 times the cell size) 70, 85, 100, and 130 µm

For more information about each instrument, see links below.


Influx cell sorter

BD Influx

The BD Influx cell sorter is a high speed jet-in-air cell sorter with analog-to-digital electronics that can detect up to 9 colours, for a total of 11 parameters.

This instrument is located inside a biosafety cabinet for sorting of human samples and other biohazardous samples. See Biosafety Policies for biosafety requirements for sorting.

General features:

Collection devices

  • Two-way sorting into microtubes, 12 x 75 mm, and 15 mL tubes
  • Six-way sorting into microtubes and 12 x 75 mm tubes
  • One-way sorting (including single cell) onto slides and into 6, 24, 96, and 384 well plates

Sample Input sizes: 12 x 75-mm tubes

Temperature Control: cooling or warming for collection tubes and sort sample

Nozzles: (nozzle should be 4-5 times the cell size). 100 µm