Publications from 2005 to present

A complete list of Dr. Howell's publications and abstracts is available on Pub Med.

2012

Broom AR, Doxey AC, Lobsanov Y, Rose DR, Howell PL, McConkey BJ, Meiering EM. Modular subdomain evolution and the computational design of a three symmetric globular proteins. Structure 20(1), 161-171, 2012. Abstract

Koo J, Burrows LL, Howell PL. Decoding the roles of pilotins and accessory proteins in secretin escort services. FEMS Microbiology Letters 328, 1-12, 2012. Abstract

Marmont LS, Whitney JC, Robinson H, Colvin KM, Parsek MR, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD. Acta Cryst. F68, 181-184, 2012. Abstract

2011

Colvan KM, Irie Y, Tart C, Urbano R, Whitney JC, Howell PL, Wosniak DJ, Parsek MR. The Pel and Psl polysaccharides provide Pseudomonas aeuruginosa structural redundancy within the biofilm matrix. Environmental Microbiology. Dec 19. doi: 10.1111/j.1462-2920.2011.02657.x. Abstract

Tammam S, Sundaram P, Koo J, Sampaleanu L, Ayers M, Chong A, Forman-Kay J, Burrows LL, Howell PL. Characterization of the PilN, PilO and PilP type IVa pilus subcomplex. Mol. Micro. 82(6), 1496-1514, 2011. Abstract

Franklin MJ, Nivens DE, Weadge JT, Howell PL. Biosynthesis of the Pseudomonas aeruginosa Exopolysaccharides alginate, Pel and Psl. Frontier in Microbiology, 2:167, 1-16, 2011. Abstract

Whitney JCC, Hay ID, Li C, Eckford PDW, Robinson H, Amaya MF, Wood LF, Ohman DE, Bear CE, Rehm BH, Howell PL. Structural basis for alginate secretin across the outer membrane PNAS, 108, 13083-88, 2011. Abstract

Wehbi H, Portillo E, Harvey H, Howell PL, Burrows LL. FimV promotes assembly of the Pseudomonas aeruginosa type IV pilus secretin. J Bacteriol. 2011 Jan;193(2):540-50. Epub 2010 Nov 19. Abstract

Siu KKW. Asmus K, Zhang N, Horvatin C, Li S, Liu T, Woods Jr VL, Howell PL. Mechanism of substrata specificity in 5'methylthioadenosine/S-adenosylhomocysteine nucleosidases. J. Struct. Biol. 173, 86-98, 2011. (Epub Jun 6, 2010) Abstract

2010

Farrar CE, Siu KKW, Howell PL, Jarrett JT. Biotin synthase exhibits burst kinetics and multiple turnovers in the absence of products and product-related biomolecules. Biochemistry,49(46), 9985-96, 2010.Abstract

Pell LG, Gasmi-Seabrook GMC, Morais M, Neudecker P, Kanelis V, Bona D, Donaldson LW, Edwards AM, Howell PL, Davidson AR, Maxwell KL. The solution structure of the C-terminal Ig-like domain of the bacteriophage λ tail tube protein. J. Mol. Biol.  403, 468-79, 2010. Abstract

Ayers M, Howell PL, Burrows LL. Complex architectures of the type IV pilus and type II secretion machineries. Future Microbiology 5,1203-18, 2010. Abstract

Weadge JT, Yip P, Robinson H, Arnett K, Tipton P. Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX. Acta Cryst., Vol F. 66(5), 588-591, 2010. Abstract

Keiski C-L, Harwich M, Jain S, Neculai AM, Whitney J, Yip P, Robinson H, Riley L, Burrows LL, Ohman D, Howell PL. AlgK is a TPR-containing protein and the periplasmic component of a novel exopolysaccharide secretin. Structure, 18(2), 265-273, 2010. Abstract

*Preview of paper was written and published in same issue of journal.
C. Whitfield and I.L. Mainprize. TPR Motifs: Hallmarks of a new polysaccharide export scaffold. Structure 18, 151-153, 2010.

2009

Ayers M, Sampaleanu LM, Koo J, Tammam S, Howell PL, Burrows LL. The Pseudomonas aeruginosa PilM/N/O/P proteins form an inner membrane complex in the type IV pilus that is required for stability of the outer membrane secretin. J. Mol. Biol.394, 128-42, 2009. Abstract

Sampaleanu LM, Bonanno JB, Ayers M, Koo J, Tammam S, Burley SK, Almo SC, Burrows LL, Howell PL. Periplasmic domains of Pseudomonas aeruginosa PilN and PilO form a stable heterodimeric complex. J. Mol. Biol.394, 143-59, 2009. Abstract

Pell LG, Liu A, Edmonds L, Donaldson LW, Howell PL, Davidson AR. The X-ray crystal structure of the phage l tail terminator protein reveals the biologically relevant hexameric ring structure and demonstrates a conserved mechanism of tail termination among all long-tailed phages.  J. Mol. Biol., 389, 939-51, 2009. Abstract

Whitney JCC, Neculai AM, Ohman DE, Howell PL. Expression, refolding crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgE. Acta Crystallagr. Vol F, 65, 463-6, 2009. Abstract

Pell LG, Kanelis V, Donaldson LW, Howell PL, Davidson AR. The structure of the phage l major tail protein reveals a common evolution for long-tailed phages and the type VI bacterial secretion system. Proc. Nat. Acad. Sci. 106(11), 4160-5, 2009. Abstract


2008

Koo J, Tammam S, Ku SY, Sampaleanu L, Burrows LL, Howell PL. PilF is an outer membrane lipoprotein required for multimerization and localization of the Pseudomonas aeruginosa type IV pilus secretin. J Bacteriol. 2008 Sep 5. [Epub ahead of print] Abstract

Zarrine-Afsar A, Wallin S, Neculai AM, Neudecker P, Howell PL, Davidson AR, Chan HS. Theoretical and experimental demonstration of the importance of specific nonnative interactions in protein folding. Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):9999-10004. Abstract

Siu KK, Lee JE, Smith GD, Horvatin-Mrakovcic C, Howell PL. Structure of Staphylococcus aureus 5'-methylthioadenosine /S-adenosylhomocysteine nucleosidase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt 5):343-50. Epub 2008 Apr 30. Abstract

Siu KK, Lee JE, Sufrin JR, Moffatt BA, McMillan M, Cornell KA, Isom C, Howell PL. Molecular determinants of substrate specificity in plant 5'-methylthioadenosine nucleosidases. J Mol Biol. 2008 Apr 18;378(1):112-28. Abstract

Lobsanov YD, Yoshida T, Desmet T, Nerinckx W, Yip P, Claeyssens M, Herscovics A, Howell PL. Modulation of activity by Arg407: structure of a fungal alpha-1,2-mannosidase in complex with a substrate analogue. Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):227-36. Abstract

Chiang P, Sampaleanu LM, Ayers M, Pahuta M, Howell PL, Burrows LL. Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU. Microbiology. 2008 Jan;154(Pt 1):114-26. Abstract

Rodinger T, Howell PL, Pomès R. Calculations of absolute protein-ligand binding free energy using distributed replica sampling. J. Chem. Phys. 129, 155102, 2008. Abstract

2007

Ku SY, Cornell KA, Howell PL. Structure of A. thaliana 5-methylthioribose kinase in complex with ADP and MTR reveals a more occluded active site than its bacterial homolog. BMC Struct Biol. 2007 Oct 25;7(1):70 [Epub ahead of print] PMID: 17961230 [PubMed - as supplied by publisher] Abstract

Keiski CL, Yip P, Robinson H, Burrows LL, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):415-8. Abstract

Tsai M, Koo J, Yip P, Colman RF, Segall ML, Howell PL. Substrate and product complexes of Escherichia coli adenylosuccinate lyase provide new insights into the enzymatic mechanism. J Mol Biol. 2007 Jul 13;370(3):541-54. Abstract

Ku SY, Yip P, Cornell KA, Riscoe MK, Behr JB, Guillerm G, Howell PL. Structures of 5-methylthioribose kinase reveal substrate specificity and unusual mode of nucleotide binding. J Biol Chem. 2007 Jul 27;282(30):22195-206. Abstract

Ku SY, Smith GD, Howell PL. ADP-2Ho as a phasing tool for nucleotide-containing proteins. Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):493-9. Abstract


2006

Smith GD, Lemke CT, Howell PL. Substructure determination in multiwavelength anomalous diffraction, single anomalous diffraction, and single isomorphous replacement with anomalous scattering data using shake-and-bake. methods Mol Biol. 2006;364:183-96. Abstract

Rodinger T, Howell PL, Pomès R. Distributed replica sampling. J. of Chem. Theory and Computation. 2(3) 725-31, 2006.

Ku S-Y, Yip P, Howell PL. Crystal structure of Escherichia coli trytophanase. Acta Cryst Vol D. D62, 814-823, 2006. Abstract

2005

Singh V, Lee JE, Nunez S, Howell PL, Schramm VL. Transition State Structure of 5'-Methylthioadenosine/S-Adenosylhomocysteine nucleosidase from Escherichia coli and its similarity to transition state analogues. Biochemistry. 2005 Sep 6;44(35):11647-11659. Abstract

Lee JE, Smith GD, Horvartin C, Huang DJT, Cornell KA, Riscoe MK, Howell PL. Structural snapshots of MTA/AdoHcy nucleosidase along the reaction coordinate provide insights into enzyme and nucleoside flexibility during catalysis. Journal of Molecular Biology, 2005 Sep 23;352(3), 559-574. Abstract

Lee JE, Luong W, Huang DJ, Cornell KA, Riscoe MK, Howell PL. Mutational analysis of a nucleosidase involved in quorum-sensing autoinducer-2 biosynthesis. Biochemistry. 2005 Aug 23;44(33):11049-11057. Abstract

Rodinger T, Howell PL, Pomes R. Absolute free energy calculations by thermodynamic integration in four spatial dimensions. J Chem Phys. 2005 Jul;123(3):34104. Abstract

Tsai M, Koo J, Howell PL. Recovery of Argininosuccinate Lyase Activity in Duck delta1 Crystallin. Biochemistry. 2005, 44(25):9034-9044. View abstract

Singh V, Evans GB, Lenz DH, Mason JM, Clinch K, Mee S, Painter GF, Tyler PC, Furneaux RH, Lee JE, Howell PL, Schramm VL. Femtomolar transition state analogue inhibitors of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Escherichia coli. J Biol Chem. 2005 May 6;280(18):18265-73. Abstract

Lee JE, Singh V, Evans GB, Tyler PC, Furneaux RH, Cornell KA, Riscoe MK, Schramm VL, Howell PL. Structural rationale for the affinity of pico- and femtomolar transition state analogues of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase. J Biol Chem. 2005 May 6;280(18):18274-82. Abstract