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About Sickkids
About SickKids

Jonathon Ditlev, PhD

Research Institute
Scientist
Molecular Medicine

University of Toronto
Assistant Professor
Biochemistry


Phone: 416-813-7654 ext. 309150
Email: jonathon.ditlev@sickkids.ca

Brief Biography

Dr. Jonathon Ditlev is a Scientist in the Molecular Medicine Program at The Hospital for Sick Children (SickKids) Research Institute. Dr. Ditlev earned his B.S. in Biology from Calvin College in 2003. Following graduation, he worked as a research assistant in the laboratory of Dr. Bin Tean Teh at the Van Andel Research Institute in Grand Rapids, Michigan. In 2012, he earned his PhD at the University of Connecticut Health Center under the advisorship of Drs. Bruce Mayer and Les Loew. Dr. Ditlev was a Ruth L. Kirschstein post-doctoral fellow at UT Southwestern Medical Center under the mentorship of Dr. Michael Rosen. He joined the SickKids Research Institute in 2019.

Academic Background

2003 - B.S., Biology, Calvin College, Grand Rapids, MI, USA

2012 - PhD, Biomedical Science, University of Connecticut Health Center, Farmington, CT, USA

Research Interests

  • Phase separation of biomolecules
  • Composition and function of biomolecular condensates
  • Membrane-localized macromolecular complexes
  • Role of biomolecular condensates in neuronal cell signaling

Achievements

  • 2014-2016: NIH Ruth L. Kirschstein National Research Service Award

Publications

Ditlev, J.A.*, Vega, A. R.*, Köster, D.V.*, Su, X., Lakoduk, A.M., Vale, R.D., Mayor, S., Jaqaman, K., Rosen, M.K. A composition-dependent molecular clutch between T cell signaling clusters and actin. ELife. 2019; 8:e42695 doi.org/10.7554/elife.42695.

Case, L.B.§, Ditlev, J.A.§, Rosen, M.K.§ Regulation of transmembrane signaling by phase separation. Annu Rev Biophys. 2019; 48: 465-494. doi.org/10.1146/annurev-biophys-052118-115534. Review.

Case, L.B., Zhang, X., Ditlev, J.A., Rosen, M.K. Stoichiometry controls activity of phase separated clusters of actin signaling proteins. Science. 2019 Mar 8; 363(6431): 1093-1097. doi.org/10.1126/science.aau6313.

Ditlev, J.A.§, Case, L.B.§, Rosen, M.K.§ Who’s in and who’s out – Compositional control of biomolecular condensates. J. Mol. Biol. 2018 Nov 2; 430(23): 4666-4684. doi.org/10.1016/j.jmb.2018.08.003. Review.

Huang, W.Y.C*, Ditlev, J.A.*, Chiang, H.-K., Rosen, M.K., Groves, J.T. Allosteric modulation of Grb2 recruitment to the intrinsically unstructured scaffold protein, LAT, by remote site phosphorylation. J Am Chem Soc. 2017 Nov 28; 139 (49): 18009-18015. doi.org/10.1021/jacs.7b09387.

Su, X., Ditlev, J.A., Rosen, M.K., Vale, R.D. Reconstitution of TCR signaling using supported lipid bilayers. Methods Mol Biol. 2017;1584:65-76. doi.org/10.1007/978-1-4939-6881-7_5.

Su, X.*, Ditlev, J.A.*, Hui, E., Xing, W., Banjade, S., Okrut, J., King, D.S., Taunton, J., Rosen, M.K., Vale, R.D. Phase separation of signaling molecules promotes T cell receptor signal transduction. Science. 2016 Apr 29; 352(6285): 595-9. doi.org/10.1126/science.aad9964.

Ditlev JA, Mayer BJ, Loew LM. There is more than one way to model and elephant. Experiment-driven modeling of the actin cytoskeleton. Biophys J. 2013 Feb 5; 104(3): 520-32. doi.org/10.1016/j.bpj.2012.12.044. Review.

Ditlev JA, Michalski PJ, Huber G, Rivera GM, Mohler WA, Loew LM, Mayer BJ. Stoichiometry of Nck-dependent actin polymerization in living cells. J. Cell Biol. 2012 May 21; 197(5): 643-658. doi.org/10.1083/jcb.201111113.

Ditlev JA, Vacanti NM, Novak IL, Loew LM. An open model of actin dendritic nucleation. Biophys J. 2009 May 6; 96(6): 3529-3542. doi.org/10.1016/j.bpj.2009.01.037.

* These authors contributed equally to this work.
§ Corresponding author