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About Sickkids
About SickKids

Jim Hu, PhD

Research Institute
Senior Scientist
Translational Medicine

University of Toronto
Professor
Department of Laboratory Medicine and Pathobiology, Paediatrics


Phone: 416-813-6412
Fax: 416-813-5771
Email: jim.hu@sickkids.ca
Alternate Contact: University of Toronto
Alternate Email: jim.hu@utoronto.ca

Brief Biography

Dr. Jim Hu is presently a Senior Scientist in the Translation Medicine Program at The Hospital for Sick Children (SickKids). He is also a Professor in the Departments of Laboratory Medicine and Pathobiology and Paediatrics and is a member of the Graduate Faculty of the Institute of Medical Science, University of Toronto.

Dr. Hu received his PhD in Biology from Harvard University. His PhD studies on RNA polymerase and gene transcription were carried out under the supervision of Dr. Lawrence Bogorad in the Department of Cellular and Developmental Biology. After graduating, Dr. Hu came to SickKids for his postdoctoral training in RNA splicing with Dr. James D. Friesen in the Department of Genetics.

Dr. Hu serves as a member of the American Society of Gene Therapy Genetic Diseases Committee (2007-2010) and as a member of the Medical/Scientific Advisory Committee of the Canadian Cystic Fibrosis Foundation and Chair of its Research Subcommittee (2006-2009). He has also served several terms as a member of the CIHR Respiratory Committee.

Research Interests

  • lung gene regulation
  • lung gene therapy
  • airway immune response

Research Activities

Development of gene therapy for airway genetic diseases

Current molecular biology and genetics technologies give us unprecedented means to identify the genetic mutations that cause diseases, to develop diagnostic methods, and to understand disease mechanisms. Research advancements have also enhanced the development of gene-based therapies for genetic lung diseases, such as cystic fibrosis (CF). Gene therapy works by replacing a gene carrying a disease-causing mutation with a healthy, functioning copy of the gene. Gene therapy has several advantages over conventional drug treatments or surgery; most importantly, it fixes the cause of a disease, not just the symptoms. Gene therapy is designed as a non-invasive treatment for infrequent use, thus reducing the drug and treatment burden on patients and improving their quality of life. Ultimately, gene therapy treatments will also be more cost-effective, reducing the financial burden on our healthcare system. Moreover, some drugs are only effective for certain gene mutations. By using a gene therapy approach, a single treatment would be able to help all patients with different mutations in the same disease-associated gene. My group has previously demonstrated that genes can be efficiently delivered to the lungs of mice, rabbits, and pigs. These technological advancements create a unique opportunity to treat lung genetic diseases like CF, by replacing disease-associated genes in the lung stem cells, which we are now directly targeting for long-term therapeutic gene expression. Discoveries made from our research will represent a major step forward in treating patients with genetic diseases of the lung, efficiently and cost-effectively.

Airway gene regulation

Many respiratory diseases, such as cystic fibrosis and asthma, involve pathological changes in lung epithelial cells. Therefore, understanding gene regulation in the lung epithelial cells has important health implications. We have been working on a set of epithelium-specific Ets-like transcription factor genes, ESE-1, ESE-2 and ESE-3, where the expression of ESE-1, in particular, is highly induced under inflammatory conditions. Using a knockout mouse model, we have shown that this gene is involved in regulating airway immune responses. We are currently elucidating its role in regulation of inflammation in other human diseases, such as sepsis and rheumatoid arthritis.

External Funding

  • Canadian Institutes of Health Research
  • Cystic Fibrosis Canada

Achievements

  • 1998-2000, Power of Dreams Research Award from Canadian Cystic Fibrosis Foundation
  • 2000-2005, Canadian Cystic Fibrosis Foundation Scholarship
  • 2004-2007, Premier’s Research Excellence Award of Ontario
  • 2005-2006, Zellers Senior Scientist Award from Canadian Cystic Fibrosis Foundation

Publications

Lee CM, Gupta S, Wang J, Johnson EM, Crofford LJ, Marshall JC, Kapoor M, Hu J (2016) Epithelium-specific Ets transcription factor-1 acts as a negative regulator of cyclooxygenase-2 in human rheumatoid arthritis synovial fibroblasts. Cell  Biosci. 6:43. doi: 10.1186/s13578-016-0105-7, PMID:27313839.

Lee CM, Wu J, Xia Y, Hu, J (2016) ESE-1 in early development: approaches for the future. Front Cell Dev Biol. 2016 Jun 28;4:73. doi: 10.3389/fcell.2016.00073, PMID:27446923.

Cao H, Wu J, Duan C, Du K, Yeger H, Hu J (2016) Long-term expression of human CFTR gene in mouse airway via HD-Ad vector delivery and transient immunosuppression. Hum Gene Ther., 27(1):83-91.

Ankathatti Munegowda M, Hu. J. (2015) Transient blocking NK cell function with small molecule inhibitors for helper dependant adenoviral vector-mediated gene delivery, Cell Biosci, 5:29. doi: 10.1186/s13578-015-0023-0, PMID: 26085921

Cao H, Ouyang H, Ip W, Du K, Duan H, Avolio J,  Wu J, Duan C,  Yeger H, Bear C, Gonska T, Hu J,  Moraes T (2015) Testing gene therapy vectors in human primary nasal epithelial cultures, Mol Ther Methods Clin Dev. 2:15034. doi: 10.1038/mtm.2015.34, PMID:26730394

Lee CM, Gupta S, Parodo J, Wu J, Marshall JC, Hu J (2015) The uncovering of ESE-1 in human neutrophils: implication of its role in neutrophil function and survival. Genes Immun, doi: 10.1038/gene.2015.10. [Epub ahead of print],  PMID:25906252

Yu Z, Xu J, Liu J, Wu J, Lee CM, Li Y, Hu J (2015). Epithelium-specific Ets-like transcription factor 1, ESE-1, regulates ICAM-1 expression in cultured lung epithelial cell lines, Mediators Inflamm, 2015:547928. doi: 10.1155/2015/547928, PMID:26185364.

Lee CM, Gupta S, Parodo J, Wu J, Marshall JC, Hu J (2015) The uncovering of ESE-1 in human neutrophils: implication of its role in neutrophil function and survival.
Genes and Immunity,  2015 Jul-Aug;16(5):356-61. doi: 10.1038/gene.2015.10. Epub 2015 Apr 23.

Yu Z, Xu J, Liu J, Wu J, Lee CM, Li Y and Hu J (2015). Epithelium-specific Ets-like transcription factor 1, ESE-1, regulates ICAM-1 expression in cultured lung epithelial cell lines, Mediators of Inflammation,  2015;2015:547928. doi: 10.1155/2015/547928. Epub 2015 Jun 21.

Yan Z, Stewart ZA, Sinn PL, Olsen JC, Hu J, McCray Jr PB, Engelhardt JF. (2015) Ferret and Pig Models of Cystic Fibrosis: Prospects and Promise for Gene Therapy. Hum Gene Ther Clin Dev. 26(1):38-49.

Xia,  E.,  Ankathatti Munegowda, M., Cao, H. and Hu. J. (2014). Lung gene therapy how to capture illumination from the light already present in the tunnel, Genes & Diseases 1 (1):40-52

Ren F, Wang R, Zhang Y, Liu C, Wang Y, Hu J, Zhang L, Chang Z. (2014) Characterization of a monoclonal antibody against CREPT, a novel protein highly expressed in tumors, Monoclon Antib Immunodiagn Immunother 33(6):401-8.
Lam, S., Cao, H., Wu, J., Duan, R. and Hu J. (2014). Highly efficient retinal gene delivery with helper-dependent adenoviral vectors. Genes & Disease. DOI: 10.1016/j.gendis. 2014.09.002

Dhingra S, Wu J, Li SH, Guo J, Huang XP, Mihic A, Hu J, Weisel RD, Li RK. (2014). Modulation of allo-immune responses by interleukin-10 prevents rejection of implanted allogeneic smooth muscle cells and restores post-infarction ventricular function, Cell Transplantation, Apr 22. doi: 10.3727/096368914X681036

Cao, H., Machuca,TN., Yeung,JC., Wu, J., Du, K., Duan, R., Hashitomo, K., Linacre, V., Coates, AL., Leung, K., Wang, J., Yeger, H., Cutz, H., Liu, L., Keshavjee, S. and Hu, J. (2013). Efficient Gene Delivery to Pig Airway Epithelia and Submucosal Glands Using Helper-dependent Adenoviral Vectors, Mol Ther Nucleic Acids, 2:e127. doi: 10.1038/mtna.2013.55.

Guan R, EI-Rass S,   Spillane D,  Lam S, Wang Y, Wu J, Chen Z, Wang A, Jia Z, Keating A, Hu J and Wen YX (2013). rbm47, a Novel RNA Binding Protein, Regulates Zebrafish Head Development. Developmental Dynamics, Dev Dyn,  242(12):1395-404.

Lee, CM and Hu, J. (2013) Cell density during differentiation can alter the phenotype of bone marrow-derived macrophages. Cell & Biosci. 3(1):30. PMID: 23895502

Xia E and Hu J. (2013) Advances in Cystic Fibrosis Gene Therapy. CML Cystic Fibrosis, 3(2):33-43.

Lu D, Wu Y, Wang Y, Ren F, Wang D, Su F, Zhang Y, Yang X, Jin G, Hao X, He D, Zhai Y, Irwin DM, Hu J, Sung JJY, Yu J, Jia B, Chang Z (2012) CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. Cancer Cell 21:92-104

Kushwah R, Oliver J, Duan R,  Zhang L, Keshavjee S and Hu J (2012). Induction of immunological tolerance towards adenoviral vectors using a novel dendritic cell-based strategy, J. Virol  2012 Jan 18 [Epub ahead of print], PMID: 22258241

Cao H, Moday RS, Hu J (2011). Gene therapy: light is finally in the tunnel. Protein Cell 2(12):973-89.

Oliver JR, Kushwah R, Hu J (2011) Multiple roles of the epithelium-specific ETS transcription factor, ESE-1, in development and disease. Lab Invest. 2011 Dec 12. doi: 10.1038/ labinvest.2011.186. [Epub ahead of print], PMID: 22157719

Kushwah R, Oliver JR, Wu J, Chang Z, Hu J. (2011) Elf3 Regulates Allergic Airway Inflammation by Controlling Dendritic Cell-Driven T Cell Differentiation. J Immunol. 2011 Sep 23. [Epub ahead of print] PMID:21948981

Kushwah R, Hu J. (2011) Role of dendritic cells in the induction of regulatory T cells. Cell Biosci. 2011 May 24;1(1):20. PMID: 21711933 [PubMed]

Oliver JR, Kushwah R, Wu J, Pan J, Cutz E, Yeger H, Waddell TK, Hu J. (2011) Elf3 plays a role in regulating bronchiolar epithelial repair kinetics following Clara cell-specific injury. Lab Invest. 2011 Jun 27. doi: 10.1038/labinvest.2011.100. [Epub ahead of print] PMID:21709667 [PubMed - as supplied by publisher]

Wu L, Lam S, Cao H, Guan R, Duan R, van der Kooy D, Bremner R, Molday RS, Hu J. (2011) Subretinal gene delivery using helper-dependent adenoviral vectors. Cell Biosci. 2011 Apr 4;1(1):15. PMID: 21711866 [PubMed]

Kushwah R, Hu J. (2011) Complexity of dendritic cell subsets and their function in the host immune system. Immunology. 2011 Aug;133(4):409-19. doi:10.1111/j.1365-2567.2011.03457.x. Epub 2011 Jun 1.Review. PMID:21627652

Cao H, Wu J, Lam S, Duan R, Newnham C, Molday RS, Graziotto JJ, Pierce EA, Hu J. (2011) Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8-implications in the pathogenesis of RP. PLoS One. 2011 Jan 19;6(1):e15860.PMID: 21283520 [PubMed - in process]

Zhou Z, Hu J, Passafaro M, Xie W, Jia Z. (2011) GluA2 (GluR2) Regulates metabotropic glutamate receptor-dependent long-term depression through N-cadherin-dependent and cofilin-mediated actin reorganization. J Neurosci. 31(3):819-833

Hu J, Wan Y. (2011) Tolerogenic dendritic cells and their potential applications. Immunology. 2011 Jan 5. doi: 10.1111/j.1365-2567.2010.03396.x. [Epub ahead of print]PMID: 21208205 [PubMed - as supplied by publisher]

Ye L, Zhu W, Backx PH, Cortez MA, Wu J, Chow YH, McKerlie C, Wang A, Tsui LC, Gross GJ, Hu J. (2010) Arrhythmia and sudden death associated with elevated cardiac chloride channel activity. J Cell Mol Med. 2010 Dec 14. doi: 10.1111/j.1582-4934.2010.01243.x. [Epub ahead of print]PMID: 21155978 [PubMed - as supplied by publisher]

Cao H, Yang T, Li XF, Wu J, Duan C, Coates AL, Hu J. (2010) Readministration of helper-dependent adenoviral vectors to mouse airway mediated via transient immunosuppression. Gene Ther. 2010 Sep 30. [Epub ahead of print]PMID: 20882053 [PubMed - as supplied by publisher]

Lee BH, Kushwah R, Wu J, Ng P, Palaniyar N, Grinstein S, Philpott DJ, Hu J (2010) Adenoviral vectors stimulate innate immune responses in macrophages through cross-talk with epithelial cells, Immunol Lett. 2010 Sep 15. [Epub ahead of print]PMID: 20850478 [PubMed - as supplied by publisher]

Yang T, Duan R, Cao H, Lee BH, Xia C, Chang Z, Tanswell AK, Hu J (2010) Development of an inflammation-inducible gene expression system using helper-dependent adenoviral vectors, J Gene Med. 2010 Sep 16. [Epub ahead of print]

Kushwah J, Hu J (2010) Dendritic cell apoptosis: regulation of tolerance versus immunity, J. Immunol. 185 (2):795-820

Chen Z, Ma X, Zhang J, Hu J, Gorczynski RM.(2010) Alternative splicing of CD200 is regulated by an exonic splicing enhancer and SF2/ASF, Nucleic Acids Res. 2010 Jun 17.  [Epub ahead of print] PMID: 20558599

Newnham CM, Hall-Pogar T, Liang S, Wu J, Tian B, Hu J, Lutz CS. (2010) Alternative polyadenylation of MeCP2: influence of cis-acting elements and trans-acting factors. RNA Biol., 7(3).  [Epub ahead of print] PMID: 20400852

Kushwah R, Wu J, Oliver JR, Jiang G, Zhang J, Siminovitch KA, Hu J.(2010) Uptake of apoptotic DC converts immature DC into tolerogenic DC, which induce differentiation of Foxp3+ regulatory T cells. Eur J Immunol. 40(4):1022-1035

Kushwah R, Oliver JR, Zhang J, Siminovitch KA, Hu J. (2009) Apoptotic dendritic cells induce tolerance in mice through suppression of dendritic cell maturation and induction of antigen-specific regulatory T cells. J. Immunol. 183(11):7104-7118

Oliver JR, Kushwah R, Wu J, Cutz E, Yeger H, Waddell TK, Hu J. (2009) Gender differences in the pulmonary regenerative response to naphthalene induced bronchiolar epithelial cell injury. Cell Prolif. 42:672-687

Wang P, Wang Q, Sun J, Wu J, Li H, Zhang N, Huang Y, Su B, Li R, Liu L, Zhang Y, Elsholtz HP, Hu J, Gaisano HY, Jin T. (2009) POU homeodomain protein Oct-1 functions as a sensor for cyclic AMP. J. Biol. Chem. 284(39):26456-26465.

Wong AP, Keating A, Lu WY, Duchesneau P, Wang X, Sacher A, Hu J, Waddell TK. (2009) Identification of a novel epithelial-like bone marrow progenitor cell population that can repopulate the airway epithelium. J. Clin. Invest. 119(2):336-348

Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z. (2009) IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling. Cell Res, 19(2):208-215.

Gonzalez-Santos JM, Cao H, Duan R, Hu, J. (2008), Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex. Hum Mol Genet.17(2):225-239.

Kushwah R, Cao H, Hu J. (2008), Characterization of pulmonary T cell response to helper-dependent adenoviral vectors following intra-nasal delivery. J. Immunol. 180:4098-4108.

Li J, Xiang YY, Ye L, Tsui LC, MacDonald JF, Hu J, Lu WY (2008), Non-steroidal anti-inflammatory drugs enhances the wild-type and delta-F508 mutant CFTR Eur. J. Respir. (PMID:18385167).

Wu J, Duan R., Cao H, Field D, Newnham CM, Koehler DR, Zamel N, Pritchard MA, Hertzog P, Post M, Tanswell A K, Hu J (2008), Regulation of epithelium-specific Ets-like factors ESE-1 and ESE-3 in airway epithelial cells: potential roles in airway inflammation. Cell Res. May 13. [Epub ahead of print] PMID: 18475289.

Ren Y, Cheng L, Rong Z, Li Z, Li Y, Zhang X, Xiong S, Hu J, Fu XY, Chang Z (2008), hSef potentiates EGF-mediated MAPK signaling through attenuating EGFR degradation. Cell Signal., 20(3):518-533.

Navab R, Pedraza C, Fallavollita L, Wang N, Chevet E, Auguste P, Jenna S, You Z, Bikfalvi A, Hu J, O'Connor R, Erickson A, Mort JS, Brodt P. (2008), Loss of responsiveness to IGF-I in cells with reduced cathepsin L expression levels. Oncogene. 2008 May 12. [Epub ahead of print]PMID: 184

Kushwah R, Oliver JR, Cao H, Hu J (2007), Nacystelyn enhances adenoviral vector-mediated gene delivery to mouse airways. Gene Ther 14(16):1243-1248.

Wong AP, Dutly AE, Sacher A, Lee H, Hwang D, Liu MY, Keshavjee S, Hu J, Waddell TK. (2007), Direct intratracheal delivery of bone marrow cells enhances engraftment in an acutely injured airway epithelium. AJP Lung Cell Mol Physiol [Epub ahead of print] PMID: 17616650

Xu J, Bai XH, Lodyga M, Han B, Xiao H, Keshavjee S, Hu J, Zhang H, Yang B, Liu M. (2007), XB130, a novel adaptor protein for signal transduction. J. Biol Chem 282(22):16401-16412.

Kushwah R, Cao H, Hu J. (2007), Potential of helper-dependent adenoviral vectors in modulating airway innate immunity. Cell Mol Immunol 4:81-89

Flotte TR, Ng P, Dylla DE, McCray PB Jr, Wang G, Kolls JK, Hu J. (2007), Viral vector-mediated and cell-based therapies for treatment of Cystic Fibrosis. Mol Ther. 15(2):229-41.

Chang Z, Babiuk LA, Hu J. (2007), Therapeutic and prophylactic potential of small interfering RNAs against severe acute respiratory syndrome: progress to date. BioDrugs. 21(1):9-15.

Koehler D, Martin B, Corey M, Palmer D, Ng P, Tanswell AK, Hu J. (2006), Readministration of helper-dependent adenovirus vectors to mouse lung. Gene Ther. 13(9):773-780

Griesenbach U, Kitson C, Garcia SE, Farley R, Singh C, Somerton L, Painter H, Smith RL, Gill DR, Hyde SC, Chow Y-H, Hu J, Gray M, Edbrooke M, Ogilvie V, MacGregor G, Scheule RK, Cheng S-H, Caplen NJ, Alton E W. (2006), Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo. Respir. Res. 2006 Feb 15;7:26. PMID: 16480492

Chang Z, Hu J. (2006), Can siRNAs conquer SARS? Gene Ther. 2006 Jun;13(11):871-2.

Farmen SL, Karp PH, Ng P, Koehler DR, Hu J, Beaudet AL, Zabner J, Welsh MJ. (2005), Transgene expression from helper-dependent adenovirus with the cytokeratin-18 promoter in differentiated human airway epithelia. Am J Physiol Lung Cell Mol Physiol. 2005 Dec;289(6):L1123-1130.

Lee H, Koehler DR, Pang CY, Levine RH, Ng P, Palmer DJ, Quinton PM, Hu J. (2005), Gene delivery to human sweat glands: a model for cystic fibrosis gene therapy. Gene Ther. 2005 Dec;12(24):1752-60.

Koehler DR, Frndova H, Leung K, Louca E, Palmer D, Ng P, McKerlie C, Cox P, Coates AL, Hu J. (2005), Aerosol delivery of an enhanced helper-dependent adenovirus formulation to rabbit lung using an intratracheal catheter. J Gene Med. 2005 Nov;7(11):1409-20.

Yaniw D, Hu J. (2005), Epithelium-specific ets transcription factor 2 upregulates cytokeratin 18 expression in pulmonary epithelial cells through an interaction with cytokeratin 18 intron 1. Cell Res. Jun;15(6):423-9.

Gonzalez-Santos JM, Cao H, Wang A, Koehler DR, Martin B, Navab R, Hu J. (2005), A complementation method for functional analysis of mammalian genes. Nucleic Acids Res. Jun 8;33(10):e94.

Cao HB, Wang A, Martin B, Koehler DR, Zeitlin PL, Tanswell AK, Hu J. (2005), Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference. Cell Res. Feb;15(2):111-9

Koehler DR, Downey GP, Sweezey NB, Tanswell AK, Hu J. (2004), Lung inflammation as a therapeutic target in Cystic Fibrosis (invited review). The American Journal of Respiratory Cell and Molecular Biology, 31:377-381.

Navab R, Gonzalez-Santos JM, Johnston MR, Liu J, Brodt P, Tsao M-S, Hu J. (2004), Enhanced invasion of human lung carcinoma cells by COUP-TFII. Cancer Research 64:5097-5105

Liu J, Blackhall F, Seiden I, Jurisica I, Navab R, Liu N, Radulovich N, Wigle D, Sultan M, Pintilie M, Hu J, Tsao M-S, Johnston M. (2004), Modeling of lung cancer by an orthotopically growing NCI-H460SM metastatic variant cell line reveals novel candidate genes for systemic metastasis. Oncogene 23:6316-6324.

Cao H, Koehler DR, Hu J. (2004), Adenoviral vectors for gene therapy (invited review). Viral Immunology 17 (3):327-333.

Koehler DR, Sajjan U, Chow Y-H, Martin B, Kent G, Tanswell AK, McKerlie C, Forstner J, Hu J. (2003), Protection of Cftr Knockout mice from acute lung infection by a helper-dependent adenoviral vector expressing Cftr in airway epithelia. Proc. Natl. Acad. Sci. USA 100: 15364-15369.

Toietta G, Koehler DR, Finegold M, Lee B, Hu J, Beaudet AL. (2003), Reduced inflammation and improved airway expression using helper-dependent adenoviral vectors with a K18 promoter. Molecular Therapy, Vol 7, No. 5:649-658.

Gonzalez-Santos JM, Wang A, Jones J, Ushida C, Liu J, Hu J. (2002), Central region of the human splicing factor Hprp3p interacts with Hprp4p. Journal of Biological Chemistry Vol 277:23764-23772.

Navab R, Wang Y, Chow Y-H, Wang A, Jankov RP, Takamoto N, Tsai SY, Tsai M-J, Tanswell AK., Hu J. (2002), Regulation of human CC10 expression by COUP-TFs. The American Journal of Respiratory Cell and Molecular Biology. 27:273-285.

Koehler DR, Hitt MM, Hu J. (2001), Challenges and strategies for cystic fibrosis lung gene therapy. Molecular Therapy, Vol. 4, No. 2, August; 84-91.

Ye L, Chan S, Chow Y-H, Tsui L-C, Hu J. (2001), Regulated expression of the human CFTR gene in epithelial cells. Molecular Therapy, Vol. 3, No. 5, May (part 1 of 2): 723-733.

Koehler DR, Hannam V, Belcastro R, Steer B, Wen Y, Post M, Downey G, Tanswell AK, Hu J. (2001), Targeting transgene expression for cystic fibrosis gene therapy. Molecular Therapy, Vol. 4, No. 1, July: 58-65

Chow Y-H, Plumb J, Wen Y, Steer B M., Lu Z, Buchwald M., Hu, J. (2000), Targeting transgene expression in tracheal submucosal glands, Prominent sites of Human CFTR expression. Molecular Therapy, 2:359-367.

Koehler DR, Chow Y-H., Plumb J, Wen Y, Rafii B, Belcastro R, Haardt M, Lukacs L, Post M., Tanswell AK, Hu J. (2000), A human epithelium-specific vector optimized in rat pneumocytes for lung gene therapy. Pediatric Research, 48:184-190.

Chow Y-H., O'Brodovich H, Plumb J, Wang Y, Hu, J. (1999), Hormonal Regulation and Genomic Structure of the Human Amiloride-sensitive Epithelial Sodium Channel alpha-subunit Gene. Pediatric Research, 46:208-214.

Heng HHQ, Wang A, Hu J. (1998), Mapping of the human HPRP3 and HPRP4 genes encoding U4-U6-associated splicing factors to chromosomes 1q21.1 and 9q31-q33. Genomics, 48: 273-275.

Chow Y-H, O'Brodovich H, Plumb J, Wen Y, Sohn K-J, Lu Z, Zhang F, Lukacs G, Tanswell AK, Hui CC, Buchwald M, Hu J. (1997), Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways. Proc. Natl. Acad. Sci. USA 94: 14695-14700.

Wang A, Forman-Kay J, Luo Y, Luo M, Chow Y-H, Plumb J, Friesen JD, Tsui L-C, Heng HHQ, Woolford JL Jr, Hu J. (1997), Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome. Human Molecular Genetics, 6: 2117-2126.

Hu J, Xu D, Schappert K, Xu Y, Friesen JD. (1995), Mutational analysis of Saccharomyces cerevisiae U4 snRNA identifies functionally important domains. Molecular and Cellular Biology, 15: 1274-1285.

Intellectual Property

Episomal Cassettes for Gene Therapy (WO 98/51807)