Developmental & Stem Cell Biology
The Arthur and Sonia Labatt Brain Tumour Research Centre
University of Toronto
Department of Molecular Genetics
Phone: 416-813-7654 ext. 309526
For more information, visit:
- Scientist, Developmental & Stem cell Biology, SickKids (2015-present)
- Damon Runyon Postdoctoral Fellow, Department of Physiology, University of California, San Francisco/Howard Hughes Medical Institute, USA (2010-2015, Advisor: Dr. Lily Jan)
- PhD, Department of Cell and Developmental Biology, Vanderbilt University, USA (2004-2010, Advisor: Dr. Chin Chiang)
- B.Sc., Biology, Xiamen University, China (2000-2004)
Ion channels are pore-forming, transmembrane proteins that regulate biological processes by controlling ion flow across cell membranes. At a cross-disciplinary field, the roles of ion channels in cancer remain underexplored. Our lab investigates the function of ion channels in brain development and cancer, using multi-disciplinary approaches including those in Drosophila genetics, mouse genetics, xenograft modeling, cell biology, and electrophysiology. We attempt to define the mechanisms that ion channels utilize to regulate neural development and tumorigenesis. We are currently investigating several questions in the lab:
- At the systematic level, what is the mutational landscape of ion channels in various cancer types, and how do recurrent ion channel mutations affect tumorigenesis?
- At the cellular level, how do ion channels regulate tumor initiation, progression and metastasis in genetically tractable animal models?
- At the molecular level, how do different ion channels form a functional network to regulate cancer cell behaviors?
- Can we use ion channel-targeting drugs for cancer treatment?
- Cancer Research Society (CRS)
- Canadian Cancer Society Research Institute (CCSRI)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- American Brain Tumor Association (ABTA)
- Concern Foundation
- Rare Diseases: Models and Mechanisms (RDMM) Network
- Meagan's Walk
- U.S. Department of Defense (DoD)
2016 - Conquer Cancer Now Award, Concern Foundation
2015 - Careers in Cancer Research Development Program New PI Award, CIHR-ICR/CCSRI
2015 - Catalyst Scholar in Stem Cell Research
2011 - Damon Runyon Cancer Research Foundation Postdoctoral Fellowship (Kandis Ann Ulrich-Carleton Fellow)
2011 - Graduate Education in Medical Sciences-Clinical and Translational Science Institute Postdoctoral Award, UCSF/HHMI
2011 - Outstanding Graduate Student Award, Department of Cell and Developmental Biology, Vanderbilt University
2010 - Chinese Government Award for Outstanding Student Abroad
Additional details including editorial highlights and media coverage can be found in the publications section of Dr. Huang's lab website.
Agnihotri S, Golbourn B, Huang X, Remke M, Younger S, Cairns RA, Chalil A, Smith CA, Krumholtz SL, MacKenzie D, Rakopoulos P, Ramaswamy V, Taccone MS, Mischel PS, Fuller GN, Hawkins C, Stanford WL, Taylor MD, Zadeh G, Rutka JT. (2016) PINK1 is a Negative Regulator of Growth and the Warburg Effect in Glioblastoma. Cancer Res. 2016 June 20. PMID: 27325644
Morrissy AS, Garzia L, Shih DJ, Zuyderduyn S, Huang X, Skowron P, Remke M, Cavalli FM, Ramaswamy V, Marra Ma, Taylor MD, et al. Divergent clonal selection dominates medulloblastoma at recurrence. Nature. 2016 Jan 13. doi: 10.1038/nature16478.
Huang X*, He Y*, Dubuc AM*, Hashizume R, Zhang W, Reimand J, Yang H, Wang TA, Stehbens S, Younger S, Barshow S, Zhu S, Cooper MK, Peacock J, Ramaswamy V, Garzia L, Wu X, Remke M, Forester CM, Kim CC, Weiss WA, James CD, Shuman MA, Bader GD, Mueller S, Taylor MD, Jan YN, Jan LY. EAG2 potassium channel with evolutionarily conserved function as a brain tumor target. (2015) Nature Neuroscience doi: 10.1038/nn.4088. (* equal contribution)
Song Y, Salegio E, Berg J, Huang X, Han C, Beattie M, Bresnahan J, Jan LY, Jan YN. (2015) The RNA splicing/repair pathway regulates CNS axon regeneration in Drosophila and mice. Nature Neuroscience. 2015 Jun;18(6):817-25. doi: 10.1038/nn.4019. Epub 2015 May 11
Hashizume R, Andor N, Ihara Y, Lerner R, Gan H, Chen X, Fang D, Huang X, Tom MW, Ngo V, Solomon D, Mueller S, Paris PL, Zhang Z, Petritsch C, Gupta N, Waldman TA, James CD. (2014) Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma. Nature Medicine. (12): 1394-1396.
Huang X# and Jan LY#. (2014) Targeting potassium channels in cancer. Journal of Cell Biology. 206(2): 151-62. (# co-corresponding authors)
Huang X, Dubuc AM, Hashizume R, Berg J, He Y, Wang J, Chiang C, Cooper MK, Northcott PA, Taylor MD, Barnes MJ, Tihan T, Chen J, Hackett CS, Weiss WA, James CD, Rowitch DH, Shuman MA, Jan YN, Jan LY. (2012) Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics. Genes & Development. 15; 26(16): 1780-96. (cover article)
Cheng FY*, Huang X*, Sarangi A, Ketova T, Cooper MK, Litingtung Y, Chiang C. (2012) Widespread contribution of Gdf7 lineage to cerebellar cell types and implications for Hedgehog-driven medulloblastoma formation. PLoS ONE. 7(4): e35541. (* equal contribution)
Huang X, Ketova T, Litingtung Y, Chiang C. (2010) Isolation, enrichment and maintenance of medulloblastoma stem cells. Journal of Visualized Experiments. (43). pii: 2086.
Huang X, Sarangi A, Ketova T, Litingtung Y, Cooper MK, Chiang C. (2010) Intracranial orthotopic allografting of medulloblastoma cells in immunocompromised mice. Journal of Visualized Experiments. (44). pii: 2153.
Huang X, Liu J, Ketova T, Fleming JT, Grover VK, Cooper MK, Litingtung Y, Chiang C. (2010) Transventricular delivery of Sonic hedgehog is essential to cerebellar ventricular zone development. Proceedings of the National Academy of Sciences USA. 107(18): 8422-7.
Huang X, Ketova T, Fleming JT, Wang H, Dey SK, Litingtung Y, Chiang C. (2009) Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexus. Development. 136(15): 2535-43.
Oh S*, Huang X*, Liu J, Litingtung Y, Chiang C. (2009) Shh and Gli3 activities are required for timely generation of motor neuron progenitors. Developmental Biology. 331(2): 261-9. (* equal contribution)
Huang X*, Goudy SL*, Ketova T, Litingtung Y, Chiang C. (2008) Gli3-deficient mice exhibit cleft palate associated with abnormal tongue development. Developmental Dynamics. 237(10): 3079-87 (* equal contribution)
Fogel JL, Chiang C, Huang X, Agarwala S. (2008) Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling. Developmental Dynamics. 237(5): 1359-72
Huang X, Litingtung Y, Chiang C. (2007) Region-specific requirement for cholesterol modification of sonic hedgehog in patterning the telencephalon and spinal cord. Development. 134(11): 2095-10
Huang X, Litingtung Y, Chiang C. (2007) Ectopic sonic hedgehog signaling impairs telencephalic dorsal midline development: implication for human holoprosencephaly. Human Molecular Genetics. 16(12): 1454-68.
Oh S, Huang X, Chiang C. (2005) Specific requirements of sonic hedgehog signaling during oligodendrocyte development. Developmental Dynamics. 234(3): 489-96.