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About Sickkids
About SickKids

Nades Palaniyar, MSc, PhD

Research Institute
Senior Scientist
Translational Medicine

University of Toronto
Associate Professor
Laboratory Medicine & Pathobiology

Full Faculty Member
School of Graduate Studies


Phone: 416-813-7654 ext. 302328
Fax: 416-813-5002
Email: nades.palaniyar@sickkids.ca

For more information, visit:

Visit the Palaniyar lab website

Brief Biography

Doctoral training

  • PhD in Molecular Biology and Genetics, University of Guelph, Canada. He studied viral nucleic acid binding proteins and DNA recombination in Dr. David Evans’ laboratory (1991-1996).

Postdoctoral training

  • Structure of the innate immune collectin surfactant protein A (SP-A) and lipids by electron microscopy (Dr. George Harauz’s laboratory at the University of Guelph, Canada 1996-1998)
  • SP-A and SP-D structure-function transgenic mouse work studies (Dr. Frank McCormack’s laboratory, University of Cincinnati, USA, 1998-2000)
  • SP-D:DNA, SP-D:antibody: decorin molecular interaction studies (Dr. Kenneth Reid’s laboratory, MRC Immunochemistry Unit, Department of Biochemistry, the University of Oxford, UK 2000-2004)

SickKids lung innate immunity research laboratory

  • Palaniyar returned to Canada in 2004 and established a laboratory to continue his research work on innate immunity that affects several lung diseases.

Research Interests

  • Link between innate and adaptive immunity – SP-D & antibodies
  • DNA, neutrophil extracellular trap (NETs) and apoptotic cell clearance

Dr. Palaniyar currently accepts graduate students and trainees.
For more information contact: nades.palaniyar@sickkids.ca

Research Activities

Infection and inflammation are major contributors to many devastating lung diseases. Dr. Palaniyar studies innate immunity, infection, inflammation and immune cell clearance in the lungs. His research over the last 13 years has brought forward many new advances in the field.

Dr. Palaniyar joined the Hospital for Sick Children as a scientist in 2004, and has continued to work on the immune functions of SP-D; the same year, neutrophil extracellular trap (NET) formation (NETosis) had been discovered as an unique form of cell death that can help to trap and kill bacteria. Dr. Palaniyar focused on developing several animal models to study pulmonary NETosis in vivo. Dr. Palaniyar’s PhD studies focused on the microbial immune evasion and genomic DNA replication. He has learned DNA biogenesis (replication, repair and recombination) and discovered novel functions of genes (DNA polymerase, helicase, ssDNA binding proteins). As a Postdoctoral Fellow he studied the structure and function of lipid associated proteins (Myelin basic protein and proteolipid protein) and innate immune proteins (pulmonary surfactant proteins SP-A and SP-D).

In Cincinnati, Dr. Palaniyar generated transgenic mice to study the functions of various domains of SP-A and SP-D. At the University of Oxford, he focused more on SP-D and discovered that SP-D and other collectins (collagenous lectins) binds effectively DNA (JBC 2004). Recently, Dr. Palaniyar was on a 6-month sabbatical at Shriners hospitals for Children - Boston to develop a pulmonary NETosis model to study the effect of burn injury on immune suppression in the lungs. The current proposal focusses on this mouse model to further understand the effect of burn injury in pulmonary immunosuppression leading to sepsis.

Dr. Palaniyar has been very interested in translating basic science findings to clinical conditions. Therefore, he have been conducting several clinically relevant studies (Roles of neutrophils and NETs in bronchiolitis obliterans and pulmonary infections in stem cell transplant children; NETs in Ex Vivo Lung Perfusion (EVLP) system, NETs in thrombomicroangiopathy/complement system, NETs in cystic fibrosis lung disease). NETs are considered to be double edge swords, and Dr. Palaniyar has been conducting pioneering research work over the last decade on this topic.

Dr. Palaniyar’s lab consists of a strong group of trainees – MSc, PhD, PDFs and team investigators to work on NETosis and SP-D. They have conceived and proved several novel ideas (functions of neutrophil mitochondria, neutrophil transcription, NETosis pathway details, relevance of NET - trapping but not effective killing of bacteria; a novel form of neutrophil death termed ApoNETosis). They have identified several candidate drugs to regulate (suppress/promote) NETosis by drug screening. Dr. Palaniyar’s lab is currently validating these drug targets. If some of these drugs become clinically useful, those compounds could be tested in the burn model (other than the DNAse enzyme). Dr. Palaniyar also has clinical and basic collaborative studies – both local and international.

Long-term Research Interests

Determining the therapeutic potential of collectins to minimize airway infection and inflammation.

For more information, visit:

http://www.lmp.utoronto.ca/graduate/prospective-graduate-students/graduate-videos-meet-our-faculty-students-and-alumni
https://www.youtube.com/watch?v=qV3-i7RRHjg
https://www.researchgate.net/profile/Nades_Palaniyar
http://www.ncbi.nlm.nih.gov/pubmed/?term=palaniyar
http://www.sickkids.ca/AboutSickKids/Newsroom/Past-News/2015/untangling-the-nets.html
http://lmp.utoronto.ca/news/news/current-news/solving-inflammation-source-qa-professor-nades-palaniyar 

https://ca.linkedin.com/in/nades-palaniyar-phd-481890aa

Conferences

  • - TASME conference (annual)
  • - iCLIIP conference (bi-annual)

Achievements

  • American Lung Association (Ohio USA) postdoctoral fellowship (1999-2000)
  • Wellcome Trust, UK/CIHR postdoctoral fellowships (2000-2004)

Publications

Last Five Years

1. Khan M., Philip, L. M., Cheung G., Vadakepeedika S., Grasemann H., Sweezey N., Palaniyar N. (2018). Regulating NETosis: Increasing pH Promotes NADPH Oxidase-Dependent NETosis (SRA). Frontiers in Medicine. 5: Article 19. http://dx.doi.org/doi: 10.3389/fmed.2018.00019.
2. Naffah de Souza C., Breda L.C.D., Khan M.A., Rogério de Almeida S., Câmara N.O.S., Sweezey N., & Palaniyar N. (2018). Alkaline pH Promotes Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Independent Neutrophil Extracellular Trap Formation: A Matter of Mitochondrial Reactive Oxygen Species Generation and Histones Citrullination and Cleavage (SRA). Frontiers in Immunology. 2018 Jan 9(8:1849). doi:10.3389/fimmu.2017.01849.
3. Cherry A., Azzouz L., Riedl M., Khan M., Pluthero F. G., Kahr W. H.A., Palaniyar N., Licht C. (2017). Relative Antibacterial Functions of Complement and NETs: NETs Trap and Complement Effectively Kills Bacteria (Co-SRA). Molecular Immunology. 2018 May;97:71-81.
4. Djiadeu P, Azzouz D, Kotra L, Sweezey N, Palaniyar N. (2017). Ultraviolet irradiation increases green fluorescence of dihydrorhodamine (DHR) 123: false-positive results for reactive oxygen species generation (SRA). Pharma Res Per. 5(2, e00303): doi:10.1002/prp2.303.
5. Djiadeu P, Kotra LP, Sweezey N, Palaniyar N. (2017). Surfactant Protein D delays Fas-and TRAIL-mediated extrinsic pathway of apoptosis in T cells (SRA). Apoptosis. February 6: doi: 10.1007/s10495-017-1348-4.
6. Djiadeu P., Farmakovski N., Azzouz D., Kotra L., Sweezey N., Palaniyar N. (2017). Surfactant Protein D Regulates Caspase-8-mediated Cascade of the Intrinsic Pathway of Apoptosis While Promoting Bleb Formation (SRA). Molecular Immunology. 2017 Dec(92): 190-198. doi:10.1016/j.molimm.2017.10.016.
7. Khan M, Farahvash A, Douda D, Licht J-C, Grasemann H, Sweezey N, Palaniyar N. (2017). JNK Activation Turns on LPS- and Gram-Negative Bacteria-Induced NADPH Oxidase-Dependent Suicidal NETosis (SRA). Sci. Rep. 7:3409. doi:10.1038/s41598-0.
8. Khan M, Palaniyar N. (2017). Transcriptional firing helps to drive NETosis (SRA). Sci. Rep. February 8: DOI:10.1038/srep417.
9. Noone DG, Riedl M, Pluthero FG, Bowman ML, Liszewski MK, Lu L, Quan Y, Balgobin S, Schneppenheim R, Schneppenheim S, Budde U, James P, Atkinson JP, Palaniyar N, Kahr WH, Licht C. (2016). Von Willebrand factor regulates complement on endothelialcells (C). Kidney Int.pii: S0085-2538(16)3: 123-134.
10. Yuen J, Pluthero FG, Douda DN, Cherry A, Riedl M, Ulanova M, Kahr WHA, Palaniyar N, Licht C. (2016). Netosing Neutrophils Activate Complement Both on Their Own NETs and Bacteria via Alternative and Non-Alternative Pathways (SRA). Front Immunol. 7: 137.
11. Riedl M., Noone D.G., Khan M.A., Pluthero F.G., Kahr W.H.A., Palaniyar N., Licht C.(2016). Complement Activation Induces Neutrophil Adhesion and Neutrophil-Platelet Aggregate Formation on Vascular Endothelial Cells (C). Kidney Int Rep. (2): 66–75. http://dx.doi.org/10.1016/j.ekir.2016.08.015.
12. Gassas A, Krueger J, Schechter T, Craig-Barnes H, Ali M, Palaniyar N. (2016). Infections and neutrophils in the pathogenesis of bronchiolitisobliterans syndrome in children after allogeneic stem cell transplantation (SRA). Pediatric Transplantation. 20(2): 303-306.
13. Douda DN, Grasemann H, Pace-Asciak C, Palaniyar N. (2015). A lipid mediator Hepoxilin A3 is a natural inducer of neutrophil extracellular traps in human neutrophils (SRA). Mediators of Inflammation. 2015(520871): 1-7.
14. Gassas A, Schechter T, Krueger J, Craig-Barnes H, Sung L, Ali M, Dell S, Egeler RM, Zaidman I, Palaniyar N. (2015). Serum Krebs Von Den Lungen-6 as a Biomarker for Early Detection of Bronchiolitis Obliterans Syndrome in Children Undergoing Allogeneic Stem Cell Transplantation (SRA). Biology of Blood and Marrow Transplantation. 21: 1524-8.
15. Ghorbani P, Santhakumar P, Hu Q, Djiadeu P, Wolever TM, Palaniyar N, Grasemann H. (2015). Shortchain fatty acids affect cystic fibrosis airway inflammation and bacterial growth (C). The European Respiratory Journal. 46(4): 1033-45.
16. Palaniyar N, Mall MA, Taube C, Worgall S, Grasemann H. (2015). New Developments in Cystic Fibrosis Airway Inflammation (CPA). Mediators Inflamm. 2015: 769425.
17. Douda DN, Khan MA, Grasemann H, Palaniyar N. SK3 channel and mitochondrial ROS mediate NADPH oxidase-independent NETosis induced by calcium influx. PNAS. 2015. 112(9):2817-22. SRA.
18. Douda DN, Khan MA, Grasemann H, Pace-Asciak, C., Palaniyar N.  A Lipid Mediator Hepoxilin A3 Is a Natural Inducer of Neutrophil Extracellular Traps in Human Neutrophils. Mediators Inflamm. 2014; 2015:520871,7. SRA.
19. Yildiz C, Palaniyar N, Otulakowski G, Khan MA, Post M, Kuebler WM, Tanswell K, Belcastro R, Masood A, Engelberts D, Kavanagh BP. Mechanical Ventilation Induces Neutrophil Extracellular Trap Formation. Anesthesiology. 2015. [Epub ahead of print]. SRA.
20. Jin L, Batra S, Douda DN, Palaniyar N and Jeyaseelan S. CXCL1 contributes to host defense in polymicrobial sepsis via modulating T cell and neutrophil functions. J Immunol. 2014; 193(7):3549-58. C.
21. Tolosa M, Palaniyar N.  Severe respiratory insufficiency during pandemic H1N1 infection: prognostic value and therapeutic potential of pulmonary surfactant protein A. Crit Care. 2014; 18(4):479. SRA.
22. Bade G, Khan MA, Srivastava AK, Khare P, Solaiappan KK, Guleria R, Palaniyar N and Talwar A. Serum cytokine profiling and enrichment analysis reveal the involvement of immunological and inflammatory pathways in stable patients with chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis. 2014; 9:759-73. CPA.
23. Dhanju R, Min W, Ackerley C, Cimpean L, Palaniyar N, Roifman CM and Grunebaum E. Pulmonary alveolar proteinosis in adenosine deaminase-deficient mice. J Allergy Clin Immunol. 2014; 133(5):1467-71. C.
24. Cote O, Clark ME, Viel L, Labbe G, Seah SY, Khan MA, Douda DN, Palaniyar N and Bienzle D. Secretoglobin 1A1 and 1A1A differentially regulate neutrophil reactive oxygen species production, phagocytosis and extracellular trap formation. PLoS One. 2014; 9(4):e96217. C.
25. Mehl, A., Ghorbani, P., Douda DN, Huang, H., Palaniyar N, Ratjen, F., Grasemann H. Effect of arginase inhibition on pulmonary L-argnine metabolism in murine Pseudomonas pneumonia. PLoS One. 2014; 9(3): e90232. C.
26. Douda DN, Yip L, Khan MA, Grasemann H and Palaniyar N.  Akt is essential to induce NADPH-dependent NETosis and to switch the neutrophil death to apoptosis. Blood. 2014; 123(4): 597-600. SRA.
27. Peter MR, Jerkic M, Sotov V, Douda DN, Ardelean DS, Ghamami N, Lakschevitz F, Khan MA, Robertson SJ, Glogauer M, Philpott DJ, Palaniyar N and Letarte M. Impaired resolution of inflammation in the Endoglin heterozygous mouse model of chronic colitis. Mediators Inflamm. 2014; 2014: 767185. C.
28. Madsen J, Gaiha GD, Palaniyar N, Dong T, Mitchell DA, Clark HW. Surfactant Protein D modulates HIV infection of both T-cells and dendritic cells. PLoS One. 2013; 8(3): e59047. C.
29. Gassas A, Craig-Barnes H, Dell SD, Cox P, Schechter T, Doyle J, Sung L, Egeler M and Palaniyar N.  Severe lung injury and lung biopsy in children post-hematopoietic stem cell transplantation: The differences between allogeneic and autologous transplantation. Pediatr Transplant. 2013; 17(3): 278-84. SRA.
30. Cheng OZ, Palaniyar N.  NET balancing: a problem in inflammatory lung diseases. Front Immunol. 2013; 4: 1. SRA.
31. Gassas A, Craig-Barnes H, Dell S, Doyle J, Schechter T, Sung L, Egeler M, Palaniyar N. Chest health surveillance utility in the early detection of bronchiolitis obliterans syndrome in children after allo-SCT. Bone Marrow Transplant. 2012; 2013; 17(3): 814-8. SRA.

Intellectual Property

Recombinant surfactant protein D compositions and methods of use thereof (WO/03/035679 A2)