Facebook Pixel Code
Rotin Lab

Related publications

Function and targets of ubiquitin ligase Nedd4

  • Attali A, Tobelaim W, Motamedchaboki K, Persaud A, Levin-Kravets O, Simpson-Lavy K-J, Pilzer I, Wolf DA, ROTIN D, Prag G.  Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.  EMBO J. (2016), In press.
  • Lu C, Thoeni C, Connor A, Kawabe H, Gallinger S, ROTIN D.  Intestinal knockout of Nedd4 enhances growth of APCmin tumors.  Oncogene (2016) 35: 1-11.
  • ZhangW, Wu K-P, Sartori MA, Kamadurai HB, Ordureau A, Jiang C, Mercredi PY, Murchie R, Hu J, Persaud A, Mukherjee M, Li N, Doye A, Walker JR, Sheng Y, Hao Z, Li Y, Brown KR, Lemichez E, Chen J, Tong Y, Harper JW, Moffat J, ROTIN D*, Schulman BA*, Sidhu SS*.  System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes.  Mol. Cell (2016) 62: 121-36. (*Co-Senior Authors)
  • Safi F, Shteiman-Kotler A, Zhong Y, Iliadi KG, Boulianne GL, ROTIN D.  Drosophila Nedd4-long reduces Amphiphysin levels in muscles and leads to impaired T-tubule formation. Mol. Biol. Cell (2016) 27: 6: 907-18.
  • Milkereit R*, Persaud A*, Vanoaica L, Guetg A, Verrey F, ROTIN D.  LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation.  Nat. Commun. (2015) 6: 7250: 1-9. (*Equal contribution)
  • Trzcinska-Daneluti AM, Chen A, Nguyen L, Murchie R, Jiang C, Moffat J, Pelletier L, ROTIN D.  RNA Interference Screen to Identify Kinases That Suppress Rescue of ΔF508-CFTR.  Mol. Cell. Proteomics (2015) 14: 1569-83.
  • Carlile GW, Robert R, Goepp J, Matthes E, Liao J, Kus B, Macknight SD, ROTIN D, Hanrahan JW, Thomas DY.  Ibuprofen rescues mutant cystic fibrosis transmembrane conductance regulator trafficking.  J. Cyst. Fibros. (2015) 14: 16-25.
  • Fang NN, Chan GT, Zhu M, Comyn SA, Persaud A, Deshaies RJ, ROTIN D, Gsponer J, Mayor T.  Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress.  Nat. Cell Biol. (2014) 16: 1227-37.
  • Persaud A, Alberts P, Mari S, Tong J, Murchie R, Maspero E, Safi F, Moran MF, Polo S, ROTIN D.  Tyrosine phosphorylation of NEDD4 activates its ubiquitin ligase activity.  Sci. Signal. (2014) 7: ra95.
  • Hsia HE, Kumar R, Luca R, Takeda M, Courchet J, Nakashima J, Wu S, Goebbels S, An W, Eickholt BJ, Polleux F, ROTIN D, Wu H, Rossner MJ, Bagni C, Rhee JS, Brose N, Kawabe H.  Ubiquitin E3 ligase Nedd4-1 acts as a downstream target of PI3K/PTEN-mTORC1 signaling to promote neurite growth.  Proc. Natl. Acad. Sci. USA (2014) 111: 13205-10.
  • Beck J, Maerki S, Posch M, Metzger T, Persaud A, Scheel H, Hofmann K, ROTIN D, Pedrioli P, Swedlow JR, Peter M, Sumara I.  Ubiquitylation-dependent localization of PLK1 in mitosis.  Nat. Cell Biol. (2013) 15: 430-9.
  • Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai AM, Neculai D, Vorobyov A, Garg P, Beatty L, Chan PK, Juang YC, Landry MC, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedadi M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, ROTIN D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS.  A strategy for modulation of enzymes in the ubiquitin system.  Science (2013) 339: 590-5.
  • ROTIN D, Staub O.  Nedd4-2 and the regulation of epithelial sodium transport.  Front. Physiol. (2012) 3: 1-7.
  • Trzcinska-Daneluti AM, Nguyen L, Jiang C, Fladd C, Uehling D, Prakesch M, Al-awar R, ROTIN D.  Use of kinase inhibitors to correct ΔF508-CFTR function.  Mol. Cell. Proteomics (2012) 11: 745-57.
  • Glowacka WK, Alberts P, Ouchida R, Wang JY, ROTIN D.  LAPTM5 protein is a positive regulator of proinflammatory signaling pathways in macrophages.  J. Biol. Chem. (2012) 287: 27691-702.
  • Nagpal P, Plant PJ, Correa J, Bain A, Takeda M, Kawabe H, ROTIN D, Bain JR, Batt JA.  The ubiquitin ligase Nedd4-1 participates in denervation-induced skeletal muscle atrophy in mice.  PLoS One (2012) 7: e46427.
  • Persaud A, Alberts P, Hayes M, Guettler S, Clarke I, Sicheri F, Dirks P, Ciruna B, ROTIN D.  Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function.  EMBO J. (2011) 30: 3259-73.
  • Kimura T, Kawabe H, Jiang C, Zhang W, Xiang YY, Lu C, Salter MW, Brose N, Lu WY, ROTIN D.  Deletion of the ubiquitin ligase Nedd4L in lung epithelia causes cystic fibrosis-like disease.  Proc. Natl. Acad. Sci. USA (2011) 108: 3216-21. (Accompanied by Press Release from APS)
  • Zhong Y, Shteiman-Kotler A, Nguyen L, Iliadi KG, Boulianne GL, ROTIN D.  A splice isoform of DNedd4, DNedd4-long, negatively regulates neuromuscular synaptogenesis and viability in Drosophila.  PLoS One (2011) 6: e27007.
  • Milkereit R, ROTIN D.  A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.  PLoS One (2011) 6: e27478.
  • ROTIN D, Staub O.  Role of the ubiquitin system in regulating ion transport.  Pflügers Arch. (2011) 461: 1-21.
  •  Alberts P, Rotin D. Regulation of lipid droplet turnover by ubiquitin ligases. BMC Biol. (2010) 8:94-6.
  • Robert R, Carlile GW, Liao J, Balghi H, Lesimple P, Liu N, Kus B, Rotin D, Wilke M, de Jonge HR, Scholte BJ, Thomas DY, Hanrahan JW. Correction of the Delta phe508 cystic fibrosis transmembrane conductance regulator trafficking defect by the bioavailable compound glafenine. Mol Pharmacol. (2010) 77:922-30
  • Fouladkou F, Lu C, Jiang C, Zhou L, She Y, Walls JR, Kawabe H, Brose N, Henkelman RM, Huang A, Bruneau BG, Rotin D. The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1. J Biol Chem. (2010) 285:6770-80
  • Persaud A, Alberts P, Amsen EM, Xiong X, Wasmuth J, Saadon Z, Fladd C, Parkinson J, Rotin D. Comparison of substrate specificity of the ubiquitin ligases Nedd4 and Nedd4-2 using proteome arrays. Mol Syst Biol. (2009) 5:333-50
  • Schimmer AD, Malkin D, Zackenhaus E, Kagal A, Klip A, Rotin D, Minden MD, Teitel J. Health care crisis in Gaza. CMAJ. (2009) 180:123
  • Kim Chiaw P, Huan LJ, Gagnon S, Ly D, Sweezey N, Rotin D, Deber CM, Bear CE. Functional rescue of DeltaF508-CFTR by peptides designed to mimic sorting motifs. Chem Biol. (2009) 16:520-30
  • Rotin D, Kumar S. Physiological functions of the HECT family of ubiquitin ligases. Nat Rev Mol Cell Biol. (2009) 10:398-409. Review
  • Trzcinska-Daneluti AM, Ly D, Huynh L, Jiang C, Fladd C, Rotin D. High-content functional screen to identify proteins that correct F508del-CFTR function. Mol Cell Proteomics. (2009) 8:780-90
  • Rotin D. Role of the UPS in Liddle syndrome. BMC Biochem. (2008) 9 Suppl 1:S5.
  • Rotin D, Schild L. ENaC and its regulatory proteins as drug targets for blood pressure control. Curr Drug Targets. (2008) 9:709-16. Review.
  • Bezsonova I, Bruce MC, Wiesner S, Lin H, Rotin D, Forman-Kay JD. Interactions between the three CIN85 SH3 domains and ubiquitin: implications for CIN85 ubiquitination. Biochemistry. (2008) 47:8937-49
  • Fouladkou F, Landry T, Kawabe H, Neeb A, Lu C, Brose N, Stambolic V, Rotin D. The ubiquitin ligase Nedd4-1 is dispensable for the regulation of PTEN stability and localization. Proc Natl Acad Sci USA. (2008) 105:8585-90
  • Bruce MC, Kanelis V, Fouladkou F, Debonneville A, Staub O, Rotin D. Regulation of Nedd4-2 self-ubiquitination and stability by a PY motif located within its HECT-domain. Biochem J. (2008) 415:155-63
  • Wiesner S, Ogunjimi AA, Wang HR, Rotin D, Sicheri F, Wrana JL, Forman-Kay JD. Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain. Cell. (2007) 130:651-62
  • Lu C, Jiang C, Pribanic S, ROTIN D. CFTR stabilizes ENaC at the plasma membrane J. Cyst. Fibros. 6:419-422, 2007.
  • Lu C, Pribanic S, Debonneville A, Jiang C, ROTIN D. The PY motif of ENaC, mutated in Liddle syndrome, regulates channel internalization, sorting and mobilization from subapical pool Traffic 8:1246-1264, 2007.
  • Ing B, Shteiman-Kotler A, Castelli M, Henry P, Pak Y, Stewart B, Boulianne GL, ROTIN D. Regulation of Commissureless by the ubiquitin ligase DNedd4 is required for neuromuscular synaptogenesis in Drosophila melanogaster. Mol. Cell. Biol. 27:481-496, 2007.
  • Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, ROTIN D. Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast. Mol. Syst. Biol. 3:116, 2007.
  • Pak Y, Glowacka WK, Bruce MC, Pham N, ROTIN D. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination J. Cell. Biol. 175:631-645, 2006.
  • Amsen EM, Pham N, Pak Y, ROTIN D. The guanine nucleotide exchange factor CNrasGEF regulates melanogenesis and cell survival in melanoma cells. J. Biol. Chem, 281:121-128, 2006.
  • Kus B, Rouleau N, Chan D, Sun W, Gajadhar A, Lenk A, Lee T, Edwards A, Bosse R, ROTIN D. A High Throughput Screen to Identify Substrates for the Ubiquitin Ligase Rsp5. J. Biol. Chem, 280:29470-29478, 2005.
  • Vecchione A, Marchese A, Henry P, ROTIN D, Morrione A. The Grb10/Nedd4 Complex Regulates Ligand-Induced Ubiquitination and Stability of the Insulin-Like Growth Factor I Receptor. Mol. Cell Biol. 23:3363-3372, 2003.
  • Henry P, Kanelis V, O'Brien C, Kim B., Gautschi I, Forman-Kay J, Schild L, ROTIN D. Affinity and specificity of interactions between Nedd4 isoforms and ENaC. J. Biol. Chem., 2003.
  • Myat A, Henry P, McCabe V, Flintoft L, ROTIN D, Tear G. Nedd4, a ubiquitin ligase, is recruited by commissureless to control cell surface levels of the roundabout receptor. Neuron 35:447-459, 2002.
  • Pak Y, Pham N, ROTIN D. Direct binding of the b1 adrenergic receptor to the cAMP dependent guanine nucleotide exchange factor CNrasGEF leads to Ras activation. Mol. Cell Biol. 22:7942-7952, 2002.
  • Pham N, ROTIN D. Nedd4 regulates ubiquitination and stability of the guanine nucleotide exchange factor CNrasGEF. J. Biol. Chem. 276:46995-47003, 2001.
  • Kanelis V, ROTIN D, Forman-Kay, J. Solution structure of a Nedd4 WW domain -bENaC peptide complex. Nature Struct. Biol. 8:407-412, 2001.
  • Plant P, Lafont F, Lecat S, Verkade P, Simons K, ROTIN D. Apical membrane localization of Nedd4 is mediated by an association of its C2 domain with Annexin XIIIb. J. Cell Biol. 149:1473-1483, 2000.
  • Pham N, Cheglakov I, Koch A, de-Hoog C, Moran MF, ROTIN D. The guanine nucleotide exchange factor CNrasGEF activates ras in response to cAMP and cGMP. Curr. Biol.10:555-558, 2000.
  • Abriel H, Loffing J, Rebhun JF, Pratt H, Schild L, Horisberger J-D, ROTIN D, Staub O. Defective regulation of the epithelial Na+ channel (ENaC) by Nedd4 in Liddle's Syndrome. J. Clin. Invest., 103:667-673, 1999.
  • Staub O, Gautschi I, Ishikawa T, Breitschopf K, Ciechanover A, Schild L, ROTIN D. Regulation of stability and function of the epithelial Na channel (ENaC) by ubiquitination. EMBO J. 16:6325-6336,1997.
  • Staub O, Dho S, Henry P, Correa J, Ishikawa T, McGlade J, ROTIN D. WW domains of Nedd4 bind to the proline rich PY motifs in the epithelial Na channel deleted in Liddle's syndrome. EMBO J, 15:2371-2380, 1996.