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SPARC BioCentre

Cell Metabolism Analysis

The SPARC BioCentre makes use of an XFe96 Extracellular Flux Analyzer from Seahorse Bioscience to interrogate in real-time the two major energy producing pathways of the cell, mitochondrial respiration and glycolysis.  XF technology simultaneously measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), such that cellular functions like oxidative phosphorylation, glycolysis, and fatty acid oxidation, and their responses to substrates, inhibitors, or other compounds can be rapidly determined in a microplate format.  The ability to measure the metabolic phenotype of cells and the shift between respiration and glycolysis under pathological conditions will allow you to connect physiological traits of cells with genomic and proteomic data.  Researchers have successfully utilized XF technology to gain new insights into diabetes, obesity, cancer, mitochondrial diseases, cardiovascular and neurodegenerative function and more.  For more information on XF technology, visit the Seahorse Bioscience website: http://www.seahorsebio.com/

As the first-step to your investigations, we recommend you perform a Glycolysis Stress Test (GST) and a Cell Mito Stress Test (MST).  These tests measure the key parameters of glycolytic and mitochondrial function, such as glycolytic capacity, basal respiration, and maximal respiration, optimize assay parameters including cell seeding density and reagent concentrations, and provide valuable information as to whether further experimentation is desirable.  Researchers who are informed on the metabolic phenotype of their cells may prefer to customize an assay by, for example, varying cell conditions, injecting different compounds, or determining dose response curves.  Wave Software from Seahorse Bioscience enables user-friendly experimental design and data interpretation, with available output formats including customizable reports and export to MS ExcelTM.

Our staff is happy to work with you during the planning and protocol development stages, and work with you to understand the results obtained.  The professional consultations are all part of the service.