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Colloidal Coomassie Blue Staining Protocol

  1. Rinse resolved gels in ultrafilter water for 5 minutes.
  2. Fix proteins with 10% acetic and 50% methanol for 10 minutes.
  3. Rinse briefly in ultrafilter water and stain for 3-12 hours in colloidal Blue stain (GelCode® blue from Pierce, cat. No. 24592 or the Colloidal Blue staining kit from Invitrogen, cat. No. LC6025)
  4. Destain for 1-7 hours in ultrafilter water.

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