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Edman Sequencing Equipment

ABI 492 Procise cLC Sequencer

 Applied Biosystems PROCISE 491C Protein Sequencing System

  • - consists of the Procise Protein Sequencer, the Model 140C Microgradient Delivery System and Perkin-Elmer Series 200 UV-VIS detector.
  • - sequentially cleaves N-terminal amino acids from protein/peptide chains and analyzes the resulting phenylthiohydantoin (PTH)-amino acid residues.
  • - capable of analyzing solubilized or PVDF-bound protein in as low as 50-100 picomole range
  • - capable of determining up to 40 residues from solubilized and very pure protein, if sufficient amount can be provided