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Training Programs

Training Programs

The mandate of the CF Canada-SickKids Program for Individualized CF therapy is to provide both resources and training for CF researchers to pursue studies on CF patient-derived induced pluripotent stem cells (iPS cells) and other CF-relevant systems to advance the goal of Personalized Medicine in CF.  

Our first training course was held May 2-5, 2017:

1. Directed Differentiation of iPS Cells into Systems to Model CF Lung Disease.  
This was a comprehensive three day workshop on methods to maintain human iPS cells in culture, and directed differentiation of these cells to epithelial cells suitable as a model for the study of Cystic Fibrosis.  In this workshop trainees had the opportunity to perform critical steps of the protocols.  We discussed and demonstrated important aspects of the workflow including characterization of patient-derived iPS cells and how they can be used as a model system for the study of CF lung disease. The workshop included lectures by world renowned researchers involved in the generation and expansion of iPS cells from blood, and in the differentiation of these cells to lung epithelia and other CF-relevant model systems. This course was opened to Canadian and international academic trainees. Limited space was available to industry participants.  

Other courses under consideration for future development:

2. Nasal cell culture and expansion. (possible future development; $ TBD)
Practical training on culturing primary nasal cells from CF donors and expanding for functional studies. Must have basic laboratory and tissue culture experience. Course is under consideration for a future offering.  

3. Functional studies on CF cell systems. (possible future development; $ TBD)
Introduction to cell-based assays for CFTR function in a variety of cell systems. Must have basic laboratory and tissue culture experience.

MBW Training Course

As part of an International effort to standardize the multiple breath washout (MBW) test, the North American MBW training centre at The Hospital for Sick Children offers an intensive MBW Training course and qualification process. The training course is designed to facilitate making real-time decisions about device operation and test quality and is delivered through a comprehensive one day hands-on session combined with two interactive online courses. MBW training information can be found here.