Paediatric Pathology is a distinct discipline that links the fundamental molecular and cellular abnormalities underlying disease processes in children to the clinical signs and symptoms of those diseases. Using techniques such as histology, cytology, morphometry, enzyme histochemistry, immunochemistry, in situ hybridization, electron microscopy and molecular biology, the pathologist pursues the diagnosis and pathogenesis of these important diseases in children. The Division of Pathology has a strong independent and collaborative research program investigating many different aspects of childhood disease, and many of our researchers have received international recognition for their work. Our educational activities cover a broad range of students, including laboratory technologists, laboratory technicians, pathologists’ assistants, pathology and neuropathology residents, and clinical fellows, as well as undergraduate teaching at the University and national webinars.
Our division performs hospital-consented autopsies, and provides a consultation service for the examination of post-mortem tissues including referred-in cases from the Coroner’s Office or outside hospitals, glass slides, organ blocks such as heart, lung or brain, or referral of fetuses or neonatal infants for a complete post-mortem examination. We provide an efficient and comprehensive consultation service to hospitals throughout Ontario, and will review selected cases from outside of this geographic area. Information about the procedure for handling specific cases is available.
Our EM facility has the capability to perform highly specialized testing and their applications to paediatric diseases, including examination of metabolic and storage diseases, examination of cilia, renal biopsy evaluation, muscle biopsy evaluation, scanning electron microscopy evaluation of hair specimens for variations caused by genetic abnormalities, evaluation of congenital enteropathies (microvillous inclusion disease, e.g.), urinary stone analysis and specialized research techniques such as cryoimmobilization and immunogold labeling.
We provide histology services which include consultations and histotechnology techniques on surgical, cytological, autopsy, veterinary and industrial research materials. This involves processing, embedding, microtomy and/or staining of paraffin-embedded and frozen tissues with a variety of specialized histochemical stains, many of which are specialized pediatric stains (acetylcholinesterase for the diagnosis of Hirschsprung disease, e.g.).
Immunohistochemistry and most recently in situ hybridization has contributed greatly to modern diagnostic pathology due to its specificity and sensitivity. For the last two decades, we have developed a comprehensive range of antibody markers and probes for immunohistochemical diagnostic service, many of which are pediatric-specific, and we develop new antibody tests regularly.
The molecular pathology laboratory in the Division of Pathology is currently expanding its test menu and incorporating several new technologies to aid in the diagnosis, prognosis and therapeutic decision making in pediatric cancers. In addition to PCR and RTPCR-based assays we now offer SNP/microarray testing of tumour tissue for medulloblastoma, low-grade glioma and neuroblastoma, which allows for the detection of gene copy number changes and variants. We are also developing NanoString® assays for medulloblastoma (subtyping into the four main molecular categories) and sarcoma (detection of diagnostically specific fusion transcripts). Both assays will be available in the fall of 2015. Further, in collaboration with molecular hematopathology, we are developing a NanoString® assay for the detection of specific fusion genes in pediatric leukemia.
We perform morphometric analysis on glass slides, photomicrographs (negatives or prints), transmission electron micrographs, and digitized images. This includes consultations, training and analysis on all types of materials, including surgical, autopsy and research specimens. This involves capturing images from raw materials, counting and measuring cells and tissue structures, plotting histograms and/or graphs, and providing final statistical analysis aided by our computerized systems.
The Neuropathology Laboratory specializes in handling surgical, autopsy and research specimens for neurosurgery, neurology, ophthalmology, orthopedics and plastic surgery.
Our expertise includes the following procedures: muscle biopsies and muscle histograms, nerve biopsies, cryotomy/cryosections, Golgi silver preparation, enzyme histochemical staining techniques, as well as processing, embedding, microtomy and staining of paraffin embedded tissue and frozen tissue. We also provide consultation and hands-on-training for most techniques including immunohistochemistry.