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Paediatric Laboratory Medicine

Bone Marrow Transplant Monitoring

Clinical Significance

Bone marrow transplantation is an important adjunct to therapy in a number of hematologic, oncologic and genetic/metabolic disorders. Following a bone marrow transplant or peripheral blood transfusion, patient hematopoietic and lymphoid cells are replaced by cells derived from the donor. The percentage of donor versus host cells is indicative of the success of the engraftment, and can be used to track, or characterize, the engraftment process. In order to define the percentage of cells from two different individuals, host and donor cells must be distinguished from each other. This is done by DNA fingerprinting. This analysis requires three specimens: 1) a source of DNA from the recipient before transplantation; 2) a source of DNA from the donor; and 3) a source of DNA from the recipient after transplantation (blood or bone marrow).

Test Name

Bone Marrow Transplant Monitoring

Test Code

BMT

Division

Molecular Genetics

Method

STR (short tandem repeat) analysis

External Proficiency Testing

CAP

Turn Around Time

1–2 weeks

Disease/Condition

Bone Marrow Transplant

Specimen Type

Blood; gDNA.

For details about specimen requirements, please refer to: Specimen Type & Requirements.

Minimum Specimen Requirements

5-10 mL EDTA or ACD 0.5 mL EDTA (neonate); minimum 10 ug in 100 uL low TE (pH8.0)

Storage/Transportation

Room Temperature

Special Requirements

Special Instructions for Genome Diagnostics Samples

Approval is not required

CPT Codes

81267

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Canada
Phone: 416-813-7200
Phone: 1-855-381-3212

Interpretation

Interpretation is provided in report