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Paediatric Laboratory Medicine

Mitochondrial Isolation

Clinical Significance

Using for Respiratory Chain Enzymes Testing

Test Name

Mitochondrial Isolation


Mitochondrial Disease


Centrifugation and Resuspension


Mitochondrial Diseases

Specimen Type

Fibroblasts and muscle

Minimum Specimen Requirements

20 dishes for mitochondrial isolation from fibroblasts; 250- 300 mg for mitochondrial isolation from fresh muscle biopsy

Optimal Collection Time

For fibroblasts: anytime
Fresh biopsy: until 2:30 pm,  Monday-Friday


For fibroblasts: Room temperature; For fresh biopsy: Transport from Pathology in dish on ice.

Special Requirements

For fresh biopsy: call to book (ext. 205989) at least 24 hours in advance for full Mitochondrial Enzyme screening on isolated mitochondria. Pick up from Pathology.

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Phone: 416-813-7200
Phone: 1-855-381-3212

Reference Range

Presence of active CS


Isolated mitochondria is used for measuring activity of respiratory chain enzymes