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Paediatric Laboratory Medicine

Pyruvate Determination

Clinical Significance

Quantitative enzymatic determination of pyruvate

Test Name

Pyruvate Determination

Alternate Name/ Synonym

  • Pyruvic Acid

Gene Name

This can indicate a respiratory chain or pyruvate metabolism, which can then be diagnosed separately

Division

Mitochondrial Disease

Method

Fluorimetric Measumements

Turn Around Time

Fibroblasts, amniocytes: 3 weeks - 3 months;

Disease/Condition

  • Lactic Acidosis
  • Pyruvate Dehydrogenase Deficiency
  • Leigh's

Specimen Type

Only for testing extracts from fibroblast and amniocyte cell cultures.

Minimum Specimen Requirements

Use one plate / flask per sample

Optimal Collection Time

For fibroblasts, amniocytes cultures: anytime

Storage/Transportation

Samples must be kept at room temperature during measurement. Extracts can be stored for up to 48hrs at -20°C.

Special Requirements

The test should be done no later than 2 weeks after the cells have been sub cultured.

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Canada
Phone: 416-813-7200
Phone: 1-855-381-3212

Reference Range

  • Fibroblasts: Reported as Lactate / Pyruvate ratio: 9.57-26.49 nmol/mg/hr

Interpretation

In the presence of excess NADH, LDH converts pyruvate to lactate. NADH absorbs light at 340nm but NAD+ does not. As NADH is oxidized to NAD+ the reduction of absorbency at 340 nm becomes a measure of the amount of pyruvate present in the fibroblast cells.