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Paediatric Laboratory Medicine

Mycoplasma PCR Cell Line testing

Test Name

Mycoplasma PCR Cell Line testing

Alternate Name/ Synonym

M. arginini, M. orale, M. hyorhinis, M. fermentans, M. hominis, M. pneumoniae, M. penetrans

Gene Name

16s RNA

Test Code

MTCPC

Division

Molecular Microbiology - Bacterial

External Proficiency Testing

Alternate assessment procedure

Turn Around Time

Within 7 days

Disease/Condition

  • Mycoplasma cell culture contamination

Specimen Type

  • Cell line preparation

Minimum Specimen Requirements

  • Cells not treated with antibiotics, must be in the second passage
  • Cells treated with antibiotics,  must be passaged at least six (6) times in antibiotic free medium
  • For a monolayer, cell confluence must be over 80%. Suspend in 5mL of growth media (antibiotic free)
  • For a suspension, cell count should be greater than 10^6 cells per mL
  • Send 2 aliquots of cells, 200 to 300 uL for each, in 1.5mL microfuge tubes
  • Cells in T25 flask will be rejected

Storage/Transportation

  • Testing is performed once weekly. Samples must be received in the Microbiology Laboratory, Room 3676, 3rd Floor Atrium , SickKids no later than 0930h Monday morning to be included in the week testing
  • When the Monday is a statutory holiday, samples submitted no later than 0930h Tuesday morning will be accepted for the week testing

Special Requirements

* Not Health Canada approved

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Canada
Phone: 416-813-7200
Phone: 1-855-381-3212