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Paediatric Laboratory Medicine

Fungal PCR

Test Name

Fungal PCR

Alternate Name/ Synonym

Pan-fungus PCR detects all fungi with the exception of Pneumocystis jirovecii

Gene Name

ITS-2 (Internal transcribed spacer region) rRNA gene

Test Code



Molecular Microbiology - Fungal


Polymerase Chain Reaction followed by DNA sequence analysis

External Proficiency Testing

College of American Pathologists

Turn Around Time

Within 7 days


  • Fungal infections
  • Fungal isolate identification

Specimen Type

  • Fresh clinical specimen
  • Fixed/paraffin embedded specimen (block, not curls)
  • Fungal isolate

Minimum Specimen Requirements

  • Sterile screw top container for  fresh clinical specimen
  • For fixed/paraffin embedded block, please send pathology report and fungal stain slides with specimen
  • Fungal isolate, please send agar slope in screw top tube or taped agar plate please indicate presumptive identification


  • Fresh specimen should be shipped on wet or dry ice
  • Room temperature for fixed/paraffin embedded specimen
  • Room temperature for fungal isolate

Special Requirements

* Not Health Canada Approved

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Phone: 416-813-7200
Phone: 1-855-381-3212