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Paediatric Laboratory Medicine

Tacrolimus (FK506)

Clinical Significance

Tacrolimus prolongs the survival of the host and transplanted graft in animal transplant models of liver, kidney, heart, bone marrow, small bowel and pancreas, lung and trachea, skin, cornea, and limb.

In animals, tacrolimus has been demonstrated to suppress some humoral immunity and, to a greater extent, cell-mediated reactions such as allograft rejection, delayed type hypersensitivity, collagen- induced arthritis, experimental allergic encephalomyelitis, and graft versus host disease.

Tacrolimus inhibits T-lymphocyte activation, although the exact mechanism of action is not known. Experimental evidence suggests that tacrolimus binds to an intracellular protein, FKBP-12. A complex of tacrolimus-FKBP-12, calcium, calmodulin, and calcineurin is then formed and the phosphatase activity of calcineurin inhibited. This effect may prevent the dephosphorylation and translocation of nuclear factor of activated T-cells (NF-AT), a nuclear component thought to initiate gene transcription for the formation of lymphokines (such as interleukin-2, gamma interferon). The net result is the inhibition of T-lymphocyte activation (i.e. immunosuppression).

Test Name

Tacrolimus (FK506)

Alternate Name/ Synonym

Prograf, FK 506

Test Code

TACRO

Drug Class

Immunosuppressive

Division

Therapeutic Drug Monitoring - TDM

Method

LC-MS/MS

External Proficiency Testing

CAP

Turn Around Time

Daily @ 10:30h

Disease/Condition

Immunosuppressive

Specimen Type

Whole Blood (EDTA) - Do not Separate

Minimum Specimen Requirements

200 uL

Optimal Collection Time

Trough: 0-30 minutes prior to dose

Storage/Transportation

4°C (transport with a cool pack if possible)

Approval is not required

CPT Codes

80197

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Canada
Phone: 416-813-7200
Phone: 1-855-381-3212

Therapeutic Range

5.0–15.0 ug/L