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Paediatric Laboratory Medicine

Insulin, Blood

Clinical Significance

Insulin is a polypeptide hormone (MW 6000) composed of two nonidentical chains, A and B, which are joined by two
disulfide bonds. Insulin is formed from a precursor, proinsulin (MW 9000), in the beta cells of the pancreas. In proinsulin, the A and B chains are joined by a connecting peptide, referred to as the C-peptide. Both insulin and C-peptide are stored in secretory granules of the islet cells of the pancreas and are then secreted. porcine insulin.Immunoassays for insulin have been widely used to provide supplementary information, first, for the diagnosis of diabetes mellitus and, second, for differential diagnosis of fasting hypoglycemia to discriminate between insulinoma and factitious hypoglycemia.

Test Name

Insulin, Blood

Test Code



Biochemistry - Biochemistry

External Proficiency Testing


Specimen Type

Serum, Sodium Heparin / Na, K EDTA / Na Fluoride plasma

Minimum Specimen Requirements

250 uL



Approval is not required

CPT Codes


Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Phone: 416-813-7200
Phone: 1-855-381-3212

Reference Range

<3m : 1-205 pmol/L (Male)
3m-11m : 6-177 pmol/L (Male)
1-6y : 8-251 pmol/L (Male)
7-10y : 11-228 pmol/L (Male)
11-18y : 26-467 pmol/L (Male)

7-10y : 11-228 pmol/L (Female)
11-18y : 26-467 pmol/L (Female)
<3m : 1-146 pmol/L (Female)
3m-11m : 4-139 pmol/L (Female)
1-6y : 8-251 pmol/L (Female)