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Paediatric Laboratory Medicine

G-band Karyotyping - Oncology, Bone Marrow Aspirate or Peripheral Blood

Clinical Significance

Pediatric hematological disorders often have recurrent chromosome changes that correlate with diagnosis and prognosis.

Test Name

G-band Karyotyping - Oncology, Bone Marrow Aspirate or Peripheral Blood

Alternate Name/ Synonym

Chromosome analysis

Gene Name

  • All chromosomes




Direct preparation and unstimulated 24 hour culture; G-banding

External Proficiency Testing


Turn Around Time

3 weeks


Pediatric Leukemia, Myelodysplastic sydrome, Aplastic anemia, Query lymphoma/solid tumour bone marrow involvement

Specimen Type

Bone Marrow Aspirate;
or Peripheral Blood

Minimum Specimen Requirements

1.5mL (bone marrow aspirate); 1-3mL (0.5 mL minimum for neonates) (peripheral blood)


Maintain at room temperature. Do not refrigerate or freeze. Ship sample overnight at room temperature for receipt at SickKids Cytogenetics Laboratory within 24 hours of collection.

Special Requirements

Collect sample in sodium heparin.

For aspirate: relocate needle prior to sampling if possible.

If the sample is a new diagnosis leukemia, and is less than the recommended volume, submit peripheral blood in sodium heparin in addition to bone marrow aspirate

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Phone: 416-813-7200
Phone: 1-855-381-3212