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Paediatric Laboratory Medicine

FISH - Paediatric Acute Lymphoblastic Leukemia Panel

Clinical Significance

ALL FISH panel will detect  most common recurrent rearrangements in pediatric B-ALL

Test Name

FISH - Paediatric Acute Lymphoblastic Leukemia Panel

Gene Name

  • ALL FISH panel: Chromosomes 4, 10, 17, ETV6-RUNX1, BCR-ABL, MYC, P16, IGH, E2A

Division

Cytogenetics

Method

Direct preparation and unstimulated 24 hour culture; FISH (Fluorescence in situ hybridization)

External Proficiency Testing

CAP
QMPLS

Turn Around Time

5-9 working days

Disease/Condition

Acute lymphoblastic leukemia, pre-B

Specimen Type

Bone marrow aspirate; or peripheral blood

Minimum Specimen Requirements

1.5 mL (bone marrow aspirate); 1-3 mL (0.5 mL minimum for neonates) (peripheral blood)

Storage/Transportation

Maintain at room temperature. Do not refrigerate or freeze. Ship sample overnight at room temperature for receipt at SickKids Cytogenetics Laboratory within 24 hours of collection.

Special Requirements

Collect sample in sodium heparin.

For aspirate: relocate needle prior to sampling if possible.

If the sample is a new diagnosis leukemia, and is less than the recommended volume, submit peripheral blood in sodium heparin in addition to bone marrow aspirate

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Canada
Phone: 416-813-7200
Phone: 1-855-381-3212

Interpretation

Provided