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Paediatric Laboratory Medicine

Medulloblastoma Molecular Subtyping (Testing for research/investigational purposes only)

Background

The tumour must have been confirmed as a medulloblastoma by a pathologist.

The tissue sent for analysis must be representative of the tumor.

Clinical Significance

Medulloblastoma subgroups are considered distinct diagnostic entities in the revised WHO classification and can be used to predict expected outcome and guide therapeutic management.

Test Name

Medulloblastoma Molecular Subtyping (Testing for research/investigational purposes only)

Gene Name

The method relies on the relative expression of 22 signature genes. Controls include three housekeeping genes.

Test Code

MP Nano MB

Division

Molecular Pathology

Method

The assay measures the expression level of 22 medulloblastoma signature genes

External Proficiency Testing

Exchange

Turn Around Time

Run once to twice each week

Disease/Condition

All samples being sent for medulloblastoma molecular subtyping must be confirmed as a medulloblastoma by a pathologist

Specimen Type

Frozen and FFPE tissues

Minimum Specimen Requirements

40 mg frozen tissue
10x10 µm paraffin sections

Optimal Collection Time

Scrolls should be shipped immediately after shaving

Storage/Transportation

FFPE scrolls and blocks can be stored at room temperature. Frozen tissue must be stored at -80˚C

Special Requirements

  1. Either frozen tissue (preferred) or Formalin-Fixed Paraffin-Embedded (FFPE) tissue may be sent.
    Note: Frozen tissue must be sent on dry ice. FFPE Scrolls and blocks can be sent at room temperature.
  2. H&E stained slide representative of the sample must also accompany tissue, scrolls or block and can be shipped at room temperature.

To avoid compromising specimen integrity, ship Monday through Wednesday only.

Do not ship specimen on the day of or the day before a Canadian statutory holiday.

Canadian Holidays to consider:

  • New Year’s Day – January 1
  • Good Friday – the Friday before Easter
  • Victoria Day – Monday on or before May 24
  • Canada Day – July 1
  • Civic Holiday – First Monday in August
  • Labour Day – First Monday in September
  • Thanksgiving – Second Monday in October
  • Christmas Day – December 25
  • Boxing Day – December 26

SNAP FROZEN TISSUES.

  • All frozen tissues must be shipped on dry ice.
  • Specimen must be sent Monday through Wednesday only.
  • Never place samples directly on the dry ice.
  • We recommend a minimum of 4 kg (approx. 10 lbs.) of dry ice for international shipments.
  • Do not over-pack. Over-packed Styrofoam will crack and dry ice will be consumed faster.
  • Regulations require that the diamond shaped dry ice sticker be placed on the outside of the shipping box.

PARAFFIN SCROLLS.

  • Scrolls from FFPE tissues can be shipped at room temperature.
  • Scrolls must be shipped immediately after shaving.

PARAFFIN BLOCKS.

  • Block(s) from FFPE tissues can be shipped at room temperature.

To avoid shipping problems:

  • To schedule a shipment, contact a freight forwarder who knows how to export medical specimens from your country.
  • Ask the forwarder to help you with local regulations, completing the necessary documentation and arranging to clear the shipment through Canada Customs.
  • Transit times may be more than one day. Please take this into account when packaging your samples.

Interpretation

Interpretation is provided in the report.