Coulter Analyzers
The facility houses a Coulter Cytomics FC500 (2-laser, 5-color) analyzer that can be found in room 2-203. The Coulter Cytomics FC500 also measures forward and side scatter.
Please see below for more information about this instrument. Further information about training and technical specifications for this instrument can be obtained from Tharsan Velauthapillai, who can be contacted at
Coulter Cytomics FC500
Instrument Configuration
| Blue 488 nm (20mW) Typical Fluorochromes |
Red 633 nm (25mW) Typical Fluorochromes |
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| Detector | λ (nm) | ||
| FL1 | 510-540 | FITC, GFP, Alexa Fluor 488 | |
| FL2 | 560-590 | PE, Cy3 | |
| FL3 | 605-630 | PE-TexasRed, ECD, PI, PE-Cy5, PerCP, PE-Cy5.5, CyChrome | |
| FL4 | 660-690 | PerCP-Cy5.5, TriColor, Quantum Red | APC, Alexa-Fluor-633, Alexa-Fluor 647 |
| FL5 | 755-785 | PE-Cy7 | APC-Cy7 |
Note: The FL4 and FL5 detectors can be used to detect fluorochromes excited by the blue or red lasers, but not both lasers simultaneously.