Coulter Analyzers
The facility houses two coulter analyzers that can be found in room 2-203. They are a Coulter Cytomics FC500 (2-laser, 5-color) and a Coulter Epics XL-MCL (1-laser, 4-colour). Both instruments also measure forward and side scatter.
Please see below for more information about each instrument. Further information about training and technical specifications for these instruments can be obtained from Tharsan Velauthapillai, who can be contacted at
Coulter Cytomics FC500
Instrument Configuration
| Blue 488 nm (20mW) Typical Fluorochromes |
Red 633 nm (25mW) Typical Fluorochromes |
||
| Detector | λ (nm) | ||
| FL1 | 510-540 | FITC, GPF, Alexa, Fluor 488 | |
| FL2 | 560-590 | PE, Cy3 | |
| FL3 | 605-630 | PE-TexasRed, ECD, PI | |
| FL4 | 660-690 | PE-Cy5, PerCP, PE-Cy5.5, CyChrome, PerCP-Cy5.5, TriColor, Quantum Red |
APC, Alexa-Fluor-633, Alexa-Fluor 647 |
| FL5 | 755-785 | PE-Cy7 | APC-Cy7 |
Note: The FL4 and FL5 detectors can be used to detect fluorochromes excited by the blue or red lasers, but not both lasers simulataneously.
Coulter Epics XL-MCL
Instrument Configuration
| Blue 488nm (20mW) Typical Fluorochromes |
||
| Detector | λ (nm) | |
| FL1 | 510-540 | FITC, GFP, Alexa Fluor 488 |
| FL2 | 560-590 | PE, Cy3 |
| FL3 | 605-630 | PE-TexasRed, ECD, PI |
| FL4 | 660-690 | PE-Cy5, PerCP, PE-Cy5.5, CyChrome, PerCP-Cy5.5, TriColor, Quantum Red |