Acknowledgment

The FMCF receives operating funds from sources who periodically ask us to cite publications reporting data from our facility. full policy

To help keep user fees low please acknowledge our contribution in your publications. Then notify us so we can document these publications.

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You will be able to access the Sorting Feedback Survey through our online reservation portal and from within sorting appointment confirmation emails.

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BD analyzers

LSRII/Cantoll after hours QC check procedure

Those who use the LSRII/Cantoll after hours, for example those who finish running their samples after 6pm Monday to Friday or those who use the instrument on weekends/holidays, are required to perform the following QC Check procedure.

Before you start running your samples

  1. Under "Shared View" in the Browser, open the "AfterHoursQC" experiment.
    LSRII - figure 1
  2. Create a new tube by clicking on the new tube icon and rename it with the date and your name. It should look like this: "yymmdd_BobSmithstart." Do not click "next tube" in the acquisition dashboard. LSRII - figure 2
  3. Run SPHERO Rainbow Calibration particles (the tubes marked with a Blue line) and record 5000 events. Do not change the voltages.

    This verifies that the instrument was functioning optimally at the beginning of your appointment.

    At TMDT in Rm 2-504 beads are located in the white fridge beside the LSRII-OICR.
    At PGCRL in Rm 18-9430 beads are located in white fridge.

    After you have finished running your samples AND completed the cleaning procedure (do not leave the room!):

  4. Acquire the calibration particles again (steps 1-3) but create a new tube and rename it following this format: "yymmdd_BobSmithend."
  5. Run water for 5 minutes and shut down the instrument. Remember to return the calibration particles to the refrigerator.
  6. If you encounter a problem, leave a note outlining what happened and what actions you took to resolve the problem and print out the QC data.

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