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BD cell sorters

AriaIII-CFI, VBYR

Aria III CFI, BYRV
Name AriaIII-CFI, VBYR
Location TMDT Room 2-203
Restricted use FMCF staff operated only
Lasers 407 nm Violet (10 mW)
488 nm Blue (20 mW)
561 nm Yellow/Green (50 mW)
633 nm Red (15 mW)
# of colours 18 colours (6V/6B+SSC/3Y/3R)
Biosafety cabinet Yes
Default configuration "New advanced configuration"

"New Advanced Configuration" (default)

LP BP Fluorochromes
407 nm, 10 mW, DPSS Violet (Coherent)
Detector A 750 LP 780/60 BV786
Detector B 690 LP 710/50 BV711
Detector C 630 LP 660/20 BV650, NC650
Detector D 595 LP 610/20 BV605, NC605
Detector E 505 LP 525/50 BV510, V500, Amcyan, Fixable Aqua
Detector F - 450/50 BV421, V450, Pacific Blue, Fixable Violet, Sytox Blue, eF450
Detector G - - No detector
Detector H - - No detector
488nm, 20 mW, Solid State Blue (Coherent)
Detector A 755 LP 780/60 PE-Cy7
Detector B 685 LP 720/40 PerCP-Cy5.5, PE-Cy5.5, PerCP-eF710
Detector C 635 LP 660/20 PE-Cy5, 7AAD, PI (660/20)
Detector D 600 LP 610/20 PE-Texas Red, PI (610/20), PE/Dazzle 594, PE-CF5-594
Detector E 556 LP 582/15 PE
Detector F 502 LP 530/30 Alexa Fluor 488, CFSE, FITC, GFP, BB515
Detector G - 488/10 SSC
Detector H - - no detector
561 nm, 50 mW, Yellow/Green DPSS (Coherent)
Detector A 755 LP 780/60 PE-Cy7-YG
Detector B 600 LP 610/20 mCherry, PE/Dazzle, PE-CF594-YG
Detector C - 582/15 DsRed, PE
633 nm, 15 mW, HeNe Red (JDS Uniphase)
Detector A 735 LP 780/60 APC-Cy7, APC-Alexa Fluor 750, APC-eF780
Detector B 685 LP 730/45 Alexa Fluor 700
Detector C - 660/20 APC, AF647