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Aria cell sorters
Aria III CFI, BYRV
| Name | Aria III CFI, BYRV |
| Location | Room 2-203 |
| Restricted use | FCF staff operated only |
| Lasers | 488 nm Blue (15 mW) 561 nm Yellow/Green (50 mW) 633 nm Red (15 mW) 405 nm Violet (10 mW) |
| # of colours | 17 colours (6B+SSC/2Y/3R/6V) |
| Biosafety cabinet | Yes |
| Default configuration | "New advanced configuration" |
"New Advanced Configuration" (default)
| 488nm 15 mW Solid State Blue (Coherent) | |||
|---|---|---|---|
| LP | BP | Fluorochromes | |
| Detector A | 755LP | 780/60 | PE-CY7 |
| Detector B | 685LP | 720/40 | PerCP-Cy5, PE-CY5.5, PerCP-eF710 |
| Detector C | 635LP | 660/20 | PE-CY5, PerCP |
| Detector D | 600LP | 610/20 | PE-Texas Red, PI, 7AAD |
| Detector E | 556LP | 576/26 | PE |
| Detector F | 502LP | 530/30 | Alexa488, CFSE, FITC, GFP |
| Detector G | - | 488/10 | SSC |
| Detector H | - | - | no detector |
| 561 nm 50 mW Yellow/Green DPSS (Coherent) | |||
| Detector A | 600LP | 610/20 | mCherry |
| Detector B | - | 582/15 | DsRed |
| Detector C | - | - | no detector |
| 633 nm 15 mW HeNe Red (JDS Uniphase) | |||
| Detector A | 755LP | 780/60 | APC-CY7, APC-Alexa750, APC-Alexa780, APC-eF780 |
| Detector B | 685LP | 730/45 | Alexa700 |
| Detector C | - | 660/20 | APC, Alexa633, Alexa647 |
| 407 nm 10 mW DPSS Violet (Coherent) | |||
| Detector A | 735LP | 780/60 | QDot 800 |
| Detector B | 685LP | 710/50 | QDot 700 |
| Detector C | 630LP | 670/14 | QDot 655 |
| Detector D | 600LP | 610/20 | QDot 605 |
| Detector E | 505LP | 525/50 | Amcyan, Indo-1 unbound |
| Detector F | - | 450/50 | DAPI, Hoescht, Cascade Blue, Pacific Blue, Alexa405 |
| Detector G | - | - | No detector |
| Detector H | - | - | No detector |
Note: PI may also be detected in Detectors B or C off the Blue laser
Note: If the Yellow/Green laser is on, a 582/15 filter is required in Detector E for detecting PE (and for running CST performance checks).
The "New Advanced Configuration" is the default cytometer configuration and was designed for a specific set of commonly used fluorochromes in which all detectors are used simultaneously. Filters in this configuration may not be optimal for your fluorochrome combination. Additional filters are available.