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Becton Dickinson (BD) analyzers

LSRII-CFI, VBGR

BD LSR II
Name LSRII-CFI, VBGR
Location PGCRL Room 18-9430
Restricted use No
Lasers 407 nm Violet (25 mW)
488 nm Blue (50 mW)
552 nm Yellow/Green (100 mW)
639 nm Red (40 mW)
# of colours 15 colours (5V/2B+SSC/5G/3R)
HTS Unit No
Default configuration "New advanced configuration 20160107"

"New Advanced Configuration 20160107" (default)

LP BP Fluorochromes
407 nm, 25 mW, DPSS Violet (Coherent)
Detector A 685 LP 710/50 BV711, QDot 705
Detector B 635 LP 660/20 BV650, QDot 655
Detector C 600 LP 610/20 BV605, QDot 605
Detector D 505 LP 525/50 BV510, V500, AmCyan, Fixable Aqua
Detector E - 450/50 BV421, V450, Pacific Blue, Fixable Violet, Sytox Blue, eF450
488nm, 50 mW Solid State Blue (Coherent)
Detector A 655 LP 710/50 PerCP-Cy5.5, PerCP-eF710
Detector B 505 LP 530/30 Alexa Fluor 488, CFSE, FITC, GFP, BB515
Detector C - 488/10 SSC
552nm, 100mW, Yellow/Green (Coherent)
Detector A 750 LP 780/60 PE-Cy7
Detector B 685 LP 720/40 PE-Cy5.5
Detector C 635 LP 660/20 PE-Cy5, 7AAD, PI (660/20)
Detector D 600 LP 610/20 PE-Texas Red, PI (610/20), PE-DAZZLE 594, PE-CF594
Detector E - 575/26 PE
Detector F - - No detector
Detector G - - No detector
Detector H - - No detector
639 nm 40 mW HeNe Red (JDS Uniphase)
Detector A 755 LP 780/60 APC-Cy7, APC-Alexa Fluor 750, APC-Alexa Fluor 780, APC-eF780
Detector B 685 LP 710/50 Alexa Fluor 700
Detector C - 660/20 APC, Alexa Fluor 633, Alexa Fluor 647

Note: For GPF, a 515/20 filter is optimal.

The "Advanced Configuration" is the default cytometer configuration and was designed for a specific set of commonly used fluorochromes in which all detectors are used simultaneously. Filters in this configuration may not be optimal for your fluorochrome combination. Additional filters are available.