Acknowledgment

The FMCF receives operating funds from sources who periodically ask us to cite publications reporting data from our facility. full policy

To help keep user fees low please acknowledge our contribution in your publications. Then notify us so we can document these publications.

Please email references to

Feedback Survey

Your feedback is the single best way to help us improve our services.

You will be able to access the Sorting Feedback Survey through our online reservation portal and from within sorting appointment confirmation emails.

We look forward to hearing from you.

Cell sorters

The facility houses five FACSArias (BD), two MoFlos (Beckman Coulter), one SH800 (Sony) and one Influx (BD). Several of our sorters are located in biosafety cabinets for sorting human samples and other biohazardous samples.

For every sort appointment clients must indicate the types of samples to be sorted and identify the Risk Group categories of any biological agents they contain. See Biosafety policies for sorting biosafety requirements.

Most sorters are operated exclusively by FMCF staff, with the exception of the Sony SH800. All new sort clients are strongly encouraged to read the Cell Sorting Guide and to speak with the sort operator before their first sort. FMCF staff can provide advice on how to prepare the sort samples and the operator can determine in advance the appropriate sorter setup.

More information

BD Cell sorters

Instrument Aria II RITT Aria II SC Aria III CFI Influx Aria Fusion
Location TMDT Room 2-504 TMDT Room 2-203 TMDT
Room 2-203
TMDT Room 2-504 TMDT Room 2-504
# of colours 11 11 17 9 16
Restricted FMCF staff operated FMCF staff operated FMCF staff
operated
FMCF staff operated FMCF staff operated
Biosafety Cabinet Yes Yes Yes Yes Yes
Lasers
Blue 20 mW 15 mW 15 mW 200 mW 50 mW
Yellow/Green - - 50 mW
(561nM Yellow/Green)
- 150mW
(532nM Green)
Red 18 mW 15 mW 15 mW 30 mW 100 mW
Violet 15 mW 10 mW 10 mW 50 mW 85 mW
UV - - - - -

Beckman Coulter sorters:

Instrument Mo Flo XDP * Mo Flo Astrios
Location PGCRL Room 18-9430 PGCRL Room 18-9430
# of colours 11-14 17
Restricted FMCF staff operated FMCF staff operated
Biosafety Cabinet Yes Yes
Lasers
Blue 200 mW 150 mW
Red 75 mW 100 mW
Yellow/Green - 150mW (Yellow 561nM)
Violet 30 mW 50 mW
UV 50 mW -

* Note: the Violet and UV laser cannot be used simultaneously on the MoFlo XDP

Sony sorter:

Instrument SH800
Location PGCRL Room 18-9430
# of colours 6
Restricted User operated
Biosafety Cabinet Yes
Lasers
Blue 30 mW
Red 30 mW
Violet 30 mW