Acknowledgment

The FMCF receives operating funds from sources who periodically ask us to cite publications reporting data from our facility. full policy

To help keep user fees low please acknowledge our contribution in your publications. Then notify us so we can document these publications.

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You will be able to access the Sorting Feedback Survey through our online reservation portal and from within sorting appointment confirmation emails.

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Sony SH800 cell sorter

Sony HC800

The Sony SH800 is a user operated benchtop sorter with digital electronics and a disposable sorting chip that can detect up to 6 colours, for a total of 8 parameters. Sort setup is fully automated and FMCF users can be trained to operate it 24/7. For more information, see training

This instrument is located inside a biosafety cabinet for sorting of human samples and other biohazardous samples. See Biosafety Policies for biosafety requirements for sorting.

General features

Collection devices

  • Two-way sorting into microtubes, 12 x 75 mm, and 15 mL tubes
  • One-way sorting (including single cell) onto slides and into multiwell plates

Sample Input sizes: 12 x 75-mm, and 15-mL tubes
Temperature Control: cooling or warming for collection tubes and sort sample
Nozzles: (nozzle should be 4-5 times the cell size): 100 µm

Name Sony SH800
Location PGCRL Room 18-9430
Restricted use User operated only
Lasers 488 nm Blue (30 mW)
638 nm Red (30 mW)
405 nm Violet (30 mW)
# of colours 6 colours (collinear lasers)
Biosafety cabinet Yes
Default configuration "Standard configuration"

"Standard Configuration" (default)

 
Detector LP BP Fluorochromes
FL1 487 450/50 PacBlue, BV421, DAPI
FL2 487 525/50 eGFP, FITC, AF488, AmCyan, BV510
FL3 561 600/60 PE, PI, BV570, BV605
FL4 685 665/30 APC, PECy5, BV650
FL5 685 720/60 AF700, PECy5.5, PerCy5.5, BV711
FL6 752 785/60 APCCy7, PECy7, BV785