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You will be able to access the Sorting Feedback Survey through our online reservation portal and from within sorting appointment confirmation emails.

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Services

Overview of services

Analytical Flow Cytometry (unassisted)

We provide access to analytical flow cytometers. Registered FMCF users must be trained by FMCF staff to run these instruments without assistance. For available instruments, see the Instruments section. For training, visit Instrument training in the 'Using the facility' section. Once trained, users can book instruments through our online booking system.

Analytical Flow Cytometry (operator assisted)

We provide operator assisted data acquisition on our analytical flow cytometers to registered FMCF users. After consultation with an operator about experimental goals and set-up, clients can bring their stained samples to the FMCF to be run be on analytical cytometer by a skilled operator. To book an assisted acquisition appointment on an analytical flow cytometer, email a completed FMCF sample acquisition form (download Word file) to Emily Reddy ().

Cell Sorting

Cell sorters are operated exclusively by Flow and Mass Cytometry Facility staff (with the exception of the Sony SH800). More information on available cell sorters is in the Instruments section. All new sort clients are strongly encouraged to read the Cell Sorting Guide and to speak with the sort operator before their first sort. FMCF staff can provide advice on how to prepare the sort samples and the operator can determine in advance the appropriate sorter setup. Sorting appointments can be booked through our online booking system. The Sony SH800 is operated exclusively by trained FMCF users and can be booked 24/7.  To learn more about training, visit our training section.

Fluidigm C1 Single Cell Capture and Genomics

We provide training and access to our Fluidigm C1 Automated Single Cell Genomics system. This system uses microfluidics to isolate 96 single cells or nuclei in picolitre-volume chambers, and allows isolation of nucleic acids from these seperated single cells. The C1 can prepare single-cell templates for qRT-PCR, RNA-seq, WGS/exome, epigenetics or miRNA expression. Validated applications include whole transcriptome surveys of cellular or tumoral heterogeneity, and discovery of new cellular populations. DNA or RNA templates generated from the C1 can be analyzed on the related Fluidigm Biomark HD and Access array systems located in The Centre for Applied Genomics. Please contact to arrange training and access.

Automated Magnetic Cell Separation

Magnetic cell separation technology (using a magnet to separate cells labelled with superparamagnetic particle conjugated antibodies) can be used to enrich heterogeneous cell samples for particular cell populations prior to sorting, or as an endpoint itself (e.g. positive selection of CD11c+ DC, generation of lineage negative cells for adoptive transfer). Our facility has two AutoMACSpro separators. Once trained, users can book instruments through our online booking system.

Helios Mass Cytometry

Mass cytometry uses metal-conjugated antibodies or other probes to comprehensively profile cellular phenotype, signaling states and cytokine production, to name just a few applications. This technique overcomes limitations of fluorescence-based flow cytometry to allow "deep" single cell profiling of up to 40 markers per sample. Thus, mass cytometry can provide a "systems-level" view of signaling and cytokine responses within the haematopoietic hierarchy and other heterogeneous cell populations. It has been used for transformative high-dimensional studies in immunology, cancer research, stem cell biology and drug response profiling. See the Helios page for more information about mass cytometry services.

We offer:

  • analytical mass cytometry (operator assisted)
  • validated mass cytometry antibodies
  • antibody metal labeling service

Data Analysis

We provide a number of data analysis related services including:

  • a flow cytometry and Helios data analysis service
  • site licenses for FlowJo data analysis software
  • access to Pro version of Cytobank data analysis platform
  • access to data analysis computers

Full details are available in our Data Analysis section.

Consultations

We provide expert consultation services to enable our users to utilize cytometry technology to enhance the scope and quality of their research. This includes assistance with experimental design/planning, panel design, optimal sample preparation, use of appropriate experimental controls, troubleshooting and protocol development. To book a consultation appointment, please contact Sheyun Zhao ()