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Rossant Lab Protocols

Whole Mount In Situs & Immunohistochemistry
  • DPERK whole-mountimmunohistochemistry
  • Combined DPERK whole-mount immunohistochemistry and in situ hybridization
  • double in situ hybridization (post-implantation)
  • double fluorescent in situ hybridization (peri-implantation)
  • whole-mount BrdU staining
  • microarrays
  • Antibody staining: early embryos Acrobat icon
  • Axiocam MR camera instructions Acrobat icon
  • Wholemount Immunohistochemistry for OPT Acrobat icon
Plasmid Maps and Sequences
  • map and sequence information :pHspLacZpA (plasmid #213)
  • hsp 68 sequence information
  • siRNA information Acrobat icon
  • siRNA map Acrobat icon
Protocols (click to download PDF) Acrobat icon
  • whole-mount in situ hybridization / Conlon version
  • combined in situ procedures / Conlon version
  • whole-mount in situ hybridization / Henrique version
  • whole-mount in situ hybridization / deRobertis version
  • clearing with BABB
  • e4.5 - 5.5 dissection protocol
  • e7.0 dissection protocol

The Hospital for Sick Children is a health care, teaching and research centre dedicated exclusively to children; affiliated with the University of Toronto.

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