Medium for XEN Cells
Xen cells may be grown in RPMI medium plus 15% FCS.
Medium:
- 500 ml RPMI + pen/strep (50 ug/ml each final conc)
- 6 ml 100 mM sodium pyruvate (GIBCO Introgen,final conc 1mM)
- 6 ml 10mM B-mercaptoethanol (Sigma, final conc 100uM)
- 6 ml 200mM L-glutamine (GIBCO Introgen,final conc 2mM)
- FCS to final volume of 15%
Medium for XEN Cells
Culture TS cells in the above medium in a standard tissue culture incubator
(37
oC, 5% CO
2 incubator. The cells are passaged (1:20
to 1:40) every 2 to 3 days. If they are split 1:20, they may be confluent in
2-3 day.The media is changed every second day; however, when they reach >60%
confluence, the media should be changed daily.