Sypro Ruby staining
SYPRO Ruby must be protected from light so cover gels during the staining process. Use plastic staining containers as glass trays will interfere with the staining process.
- Fix resolved gel(s) in 10% Acetic acid and 40% methanol for 1 hour.
- Incubate fixed gel(s) in SYPRO Ruby gel stain (Molecular Probes) for 4-12 hours.
- Destain the gel(s) in 7% acetic acid and 10% methanol for the same length of time used to stain the gel(s).
- Gels can be stored in the destain solution or water at 4ºC.
- Proteins in the gel are visible by a 300nm UV, blue-light transilluminator, or a laser scanner.
We recommend that you run multiple lanes and submit several bands together, excise bands with as little excess gel as possible, and take a picture of the stained gel and submit it along with your samples.