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Paediatric Laboratory Medicine

Bartonella group PCR (B. henselae, B. quintana, B. bacilliformis, B. clarridgeiae, B. elizabethae)

Test Name

Bartonella group PCR (B. henselae, B. quintana, B. bacilliformis, B. clarridgeiae, B. elizabethae)

Alternate Name/ Synonym

Bartonellosis, endocarditis, cat scratch disease

Gene Name

Riboflavin synthase

Test Code

BARPC

Division

Molecular Microbiology - Bacterial

Method

Polymerase Chain Reaction

External Proficiency Testing

Alternate assessment procedure

Turn Around Time

Within 4 days

Disease/Condition

B. henselae:

  • Cat scratch disease
  • Bacillary angiomatosis
  • Peliosis hepatitis
  • Endocarditis                                               

B. quintana:

  • Trench fever
  • Bacillary angiomatosis
  • Endocarditis

Specimen Type

  • CSF
  • Lymph node biopsy or aspirate
  • Tissue or biopsy
  • Whole blood

Minimum Specimen Requirements

  • CSF 300 ul per 1 test, for multiple tests please ensure adequate sample volume is submitted
  • Sterile screw top container for tissues, biopsies or aspirates
  • Whole blood (EDTA) - 1 mL minimum for 1 test, 3-5 mL recommended for multiple tests

Storage/Transportation

  • All specimens must be shipped on dry ice.
  • Exception: Specimens that will arrive at SickKids within 24 hours from the time of collection can be shipped on ice packs

Special Requirements

* Not Health Canada approved

Shipping and Contact Information

The Hospital for Sick Children
Rapid Response Laboratory
170 Elizabeth Street, Room 3642
Toronto, ON
M5G 2G3
Canada
Phone: 416-813-7200
Phone: 1-855-381-3212