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Becton Dickinson (BD) analyzers


Location PGCRL, Room 18-9430
Restricted use No
Lasers 407 nm Violet (25 mW)
488 nm Blue (100 mW)
633 nm Red (20 mW)
# of colours 12 colours (3V/6B+SSC/3R)
HTS unit Yes
Default configuration "New advanced configuration 04012016"

"New Advanced Configuration 04012016" (default)

407 nm, 25 mW, HeNe DPSS Violet (Coherent)
Detector A 600 LP 610/20 BV605, Qdot 605
Detector B 505 LP 525/50 BV510, AmCyan, Fixable Aqua
Detector C   450/50 BV421, Pacific Blue, Fixable Violet, Sytox Blue, eF450
488 nm, 100 mW, Solid State Blue (Coherent)
LP BP Fluorochromes
Detector A 755 LP 780/60 PE-Cy7
Detector B 685 LP 720/40 PerCP-Cy5.5, PE-Cy5.5, PerCP-eF710
Detector C 635 LP 660/20 PE-Cy5, 7AAD, PI (660/20)
Detector D 600 LP 610/20 PE-Texas Red, PI (610/20), PE-CF594, PE/Dazzle 594
Detector E 550 LP 575/26 PE
Detector F 505 LP 530/30 Alexa Fluor 488, CFSE, FITC, GFP, BB515
Detector G - 488/10 SSC
Detector H - - No detector
633 nm, 20 mW HeNe Red (JDS Uniphase)
Detector A 755 LP 780/60 APC-Cy7, APC-Alexa Fluor 750, APC-Alexa Fluor 780, APC-eF780
Detector B 685 LP 710/50 Alexa Fluor 700
Detector C   660/20 APC, Alexa Fluor 633, Alexa Fluor 647

Note: For GFP, a 515/20 is optimal

The "Advanced Configuration" is the default cytometer configuration and was designed for a specific set of commonly used fluorochromes in which all detectors are used simultaneously. Filters in this configuration may not be optimal for your fluorochrome combination. Additional filters are available.