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Becton Dickinson (BD) analyzers

LSRII-OICR, VBGR

BD LSR II
Name LSR II OICR, VBGR
Location TMDT Room 2-504
Restricted use No
Lasers 407 nm Violet (50 mW)
488 nm Blue (50 mW)
532 nm Green (200 mW)
640 nm Red (40 mW)
# of colours 18 colours (8V/2B+SSC/5G/3R)
HTS unit No
Default configuration "New advanced configuration 20160108"

"New Advanced Configuration 20160108 (default)

LP BP Fluorochromes
407 nm, 50 mW, DPSS Violet (Coherent)
Detector A 735 LP 780/60 BV786, Qdot 800
Detector B 685 LP 710/50 BV711, Qdot 705
Detector C 635 LP 660/20 BV650, Qdot 655
Detector D 600 LP 610/20 BV605, Qdot 605
Detector E 570 LP 585/42 Qdot 585
Detector F 545 LP 560/40 Pacific Orange
Detector G 505 LP 525/50 BV510, AmCyan, Fixable Aqua
Detector H - 450/50 BV421, Pacific Blue, Fixable Violet, Sytox Blue, eF450
488nm, 50 mW, Solid State Blue (Coherent)
Detector A 685 LP 710/50 PerCP-Cy5.5, PerCp-eF710
Detector B 505 LP 515/20 Alexa Fluor 488, CFSE, FITC, GFP, BB515
Detector C - 488/10 SSC
532 nm, 200 mW, Green (Coherent)
Detector A 755 LP 780/60 PE-Cy7
Detector B 685 LP 720/40 PECy5.5
Detector C 635 LP 660/20 PECy5, 7AAD, PI (660/20)
Detector D 600 LP 610/20 PE-Texas Red, PI (610/20), PE/Dazzle 594, PE-CF594
Detector E - 575/26 PE
Detector F - - No detector
Detector G - - No detector
Detector H - - No detector
633 nm, 40 mW, HeNe Red (JDS Uniphase)
Detector A 755 LP 780/60 APC-Cy7, APC-Alexa Fluor750, APC-Alexa Fluor 780, APC-eF780
Detector B 685 LP 710/50 Alexa Fluor 700
Detector C - 660/20 APC, Alexa Fluor 633, Alexa Fluor 647

Note: For GFP, a 515/20 filter is optimal.

The "Advanced Configuration" is the default cytometer configuration and was designed for a specific set of commonly used fluorochromes in which all detectors are used simultaneously. Filters in this configuration may not be optimal for your fluorochrome combination. Additional filters are available.