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The SickKids-UHN Flow Cytometry Facility at TMDT
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  • Instruments
    • BD analyzers
      • LSR II CFI, BGRV
      • LSR II GC, BRVU
      • LSR II OICR, BGRV
      • LSR II SC, BRVU
      • LSRFortessa, BYRVU
      • Canto II, BRV
      • Canto II FCF, BRV
      • Additional filters
      • General policies / after hours
        • LSRII QC check procedure
      • Troubleshooting
    • Coulter analyzer
    • Cell sorters
      • Aria sorters
        • Aria II GC, BRU
        • Aria II RITT, BRV
        • Aria II SC, BRV
        • Aria III CFI, BYRV
      • Beckman Coulter sorters
        • MoFlo, BRU
        • MoFlo Z-configuration
        • MoFlo XDP, BR(V/U)
        • MoFlo XDP configuration
      • Cell sorting guide
    • Magnetic cell separators
    • Data analysis platforms
      • Data transfer protocol
  • Tools and resources
    • Basic protocols
      • DNA/PI staining for cell cycle
      • Extracellular staining protocol
      • Fixing stained cells
      • Intracellular staining protocol
      • Forward and side scatter optimization
      • Optimizing PMT voltages
        • Shortcuts
        • Protocol
    • Laser / Filter combinations
    • Fluorochrome table
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Policies

  • Booking policies
  • Biosafety policies
  • BD Analyzers general rules and data storage
  • Facility acknowledgment policy
  • FCF Data Transfer protocol

The Hospital for Sick Children is a health care, teaching and research centre dedicated exclusively to children;
affiliated with the University of Toronto. For general inquires please call: 416-813-1500.

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