Pediatric Low Grade Glioma Fusion Transcript Panel

Alternate test name

glioma; low grade glioma; PLGG; LGG; pilocytic astrocytoma; astrocytoma; cancer; pediatric; pediatric brain tumor

Gene name / Alternate gene name

Refer to link on requisition

Lab area

Pathology - Molecular

Method and equipment

nanoString nCounter Technology

Expected turn-around time

7 Business Days

Specimen type

Tumour Tissue - 40 mg frozen tissue, or 10x10 FFPE micron sections, or FFPE block

Specimen requirements

The tissue provided must be representative of the tumour
• One H&E stained slide representative of the tested tissue should be provided
• RNA-based assay

Storage and transportation

To avoid compromising specimen integrity, ship Monday through Wednesday only.
Do not ship specimen on the day of or the day before a Canadian statutory holiday.
Canadian Holidays to consider:
• New Year’s Day – January 1
• Good Friday – the Friday before Easter
• Victoria Day – Monday on or before May 24
• Canada Day – July 1
• Civic Holiday – First Monday in August
• Labour Day – First Monday in September
• Thanksgiving – Second Monday in October
• Christmas Day – December 25
• Boxing Day – December 26

SNAP FROZEN TISSUES.
• All frozen tissues must be shipped on dry ice.
• Specimen must be sent Monday through Wednesday only.
• Never place samples directly on the dry ice.
• We recommend a minimum of 4 kg (approx. 10 lbs.) of dry ice for international shipments.
• Do not over-pack. Over-packed Styrofoam will crack and dry ice will be consumed faster.
• Regulations require that the diamond shaped dry ice sticker be placed on the outside of the shipping box.

PARAFFIN SCROLLS.
• Scrolls from FFPE tissues can be shipped at room temperature.
• Scrolls must be shipped immediately after shaving.

PARAFFIN BLOCKS.
• Block(s) from FFPE tissues can be shipped at room temperature.

To avoid shipping problems:
• To schedule a shipment, contact a freight forwarder who knows how to export medical specimens from your country.
• Ask the forwarder to help you with local regulations, completing the necessary documentation and arranging to clear the shipment through
Canada Customs.
• Transit times may be more than one day. Please take this into account when packaging your samples.

Requisition

Pediatric Low Grade Glioma Fusion Transcript Panel - requisition

Background and clinical significance

This panel identifies the most common fusions found in pediatric low grade glioma including BRAF and FGFR1