TruSight RNA Pan-Cancer Panel - Tissue

Alternate test name

cancer; pediatric; pediatric brain tumor; adolescent cancer; adult cancer; AYA; fusion panel; NGS panel; RNAseq

Gene name / Alternate gene name

1385 oncology genes.
For the complete gene list, refer to link on requisition

Lab area

Pathology - Molecular

Method and equipment

RNA-based assay - TruSight RNA Pan-Cancer Panel (Illumina, Inc.)

Expected turn-around time

10 Business Days

Specimen type

Tumour Tissue - 40 mg of frozen tissue or 10 x 10 FFPE micron sections or FFPE Block

Specimen requirements

The tissue provided must be representative of the tumour
• One H&E stained slide representative of the tested tissue should be provided
• RNA based assay

Storage and transportation

To avoid compromising specimen integrity, ship Monday through Wednesday only.
Do not ship specimen on the day of or the day before a Canadian statutory holiday.
Canadian Holidays to consider:
• New Year’s Day – January 1
• Good Friday – the Friday before Easter
• Victoria Day – Monday on or before May 24
• Canada Day – July 1
• Civic Holiday – First Monday in August
• Labour Day – First Monday in September
• Thanksgiving – Second Monday in October
• Christmas Day – December 25
• Boxing Day – December 26

SNAP FROZEN TISSUES.
• All frozen tissues must be shipped on dry ice.
• Specimen must be sent Monday through Wednesday only.
• Never place samples directly on the dry ice.
• We recommend a minimum of 4 kg (approx. 10 lbs.) of dry ice for international shipments.
• Do not over-pack. Over-packed Styrofoam will crack and dry ice will be consumed faster.
• Regulations require that the diamond shaped dry ice sticker be placed on the outside of the shipping box.

PARAFFIN SCROLLS.
• Scrolls from FFPE tissues can be shipped at room temperature.
• Scrolls must be shipped immediately after shaving.

PARAFFIN BLOCKS.
• Block(s) from FFPE tissues can be shipped at room temperature.

To avoid shipping problems:
• To schedule a shipment, contact a freight forwarder who knows how to export medical specimens from your country.
• Ask the forwarder to help you with local regulations, completing the necessary documentation and arranging to clear the shipment through
Canada Customs.
• Transit times may be more than one day. Please take this into account when packaging your samples.

Special requirements

Shipping information

The Hospital for Sick Children

Division of Pathology

3rd Floor, Burton Wing, 555 University Avenue, Room 3110

Toronto, ON

Canada

M5G 1X8

Phone: 416-813-5967

Hours: Monday to Friday, 8 a.m. to 5 p.m.

Off hours: Please send to Rapid Response Laboratory, 555 University Avenue, Room 3642

Requisition

TruSight RNA Pan-Cancer Panel - Tissue - requisition

Background and clinical significance

This panel targets 1385 cancer genes for variant, and fusion detection studies in many RNA sample types including fresh/frozen tissue, blood, bone marrow, and formalin-fixed, paraffin-embedded (FFPE) tissue

Disease condition