Creatinine, fluid

Equipment : Roche Cobas Pro c503

Method : This enzymatic method is based on the conversion of creatinine with the aid of creatininase, creatinase, and sarcosine oxidase to glycine, formaldehyde
and hydrogen peroxide. Catalyzed by peroxidase the liberated hydrogen peroxide reacts with 4‑aminophenazone and HTIBa) to form a quinone imine chromogen. The color intensity of the quinone imine chromogen formed is directly proportional to the creatinine concentration in the reaction mixture. Creatine of the sample is destroyed by creatinase, SOD and catalase during incubation in R1.