Lipase, plasma or serum

Alternate test name

Pancreatic lipase

Lab area

Clinical Biochemistry - General

Method and equipment

Equipment : Roche Cobas Pro c503

Method : Enzymatic colorimetric assay with 1,2‑O‑dilauryl-rac-glycero-3-glutaric-acid-(6‑methylresorufin) ester as substrate. The chromogenic lipase substrate 1,2‑O‑dilauryl-rac-glycero-3-glutaric-acid-(6‑methylresorufin) ester is cleaved by the catalytic action of alkaline lipase solution to form 1,2‑O‑dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6‑methylresorufin) ester. This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. Addition of detergent and colipase increases the specificity of the assay for pancreatic lipase. The color intensity of the red dye formed is directly proportional to the lipase activity and can be determined photometrically.

Expected turn-around time

STAT: 2 Hours Urgent: 4 Hours Routine: 24 Hours

Specimen type

Serum, Heparin Plasma

Specimen requirements

0.5 mL

Storage and transportation

Send Frozen

Special requirements

Specimen should be sent frozen.

Shipping information

The Hospital for Sick Children

Rapid Response Laboratory

555 University Avenue, Room 3642

Toronto, ON

Canada

M5G 1X8

Phone: 416-813-7200

Toll Free: 1-855-381-3212

Hours: 7 days/week, 24 hours/day

Requisition

Lipase, plasma or serum - requisition

Background and clinical significance

Pancreatic lipase in serum and plasma is closely associated with pancreatic diseases. The activity of this enzyme has been measured as an important marker for diagnosing pancreatic diseases and the associated monitoring of therapeutic effects.